BMRB Entry 18111

Name: Structure of T7 transcription factor Gp2-E. coli RNAp jaw domain complex
Published: 2012-01-25

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PDB ID: 2lmc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18111
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of T7 transcription factor Gp2-E. coli RNAp jaw domain complex

Deposition date:

2011-11-29

Original release date:

2012-01-25

Authors:

Liu, Minhao

Citation:

Citation: Liu, Minhao; Matthews, Stephen; James, Ellen; Sheppard, Carol. "Structure of T7 transcription factor Gp2-E. coli RNAp jaw domain complex" Mol. Cell ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 84 residues, 6635.446 Da.
entity_2, polymer, 84 residues, 6795.774 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 10760 Superkingdom: not available Kingdom: not available Genus/species: Bacteriophage T7

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MSNVNTGSLSVDNKKFWATV ESSEHSFEVPIYAETLDEAL ELAEWQYVPAGFEVTRVRPC VAPK
entity_2: MGSSHHHHHHSSGLVPRGSH MKEPAILAEISGIVSFGKET KGKRRLVITPVDGSDPYEEM IPKWRQLNVFEGERVERGDV ISDG

Data sets:

assigned_chemical_shifts
- Bacteriophage_T7_Gp2

Data type	Count
13C chemical shifts	499
15N chemical shifts	113
1H chemical shifts	762

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	2

Entities:

Entity 1, entity_1 84 residues - 6635.446 Da.

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

SER

ASN

VAL

ASN

THR

GLY

SER

LEU

SER

4

VAL

ASP

ASN

LYS

PHE

TRP

ALA

THR

VAL

5

GLU

SER

GLU

HIS

SER

PHE

GLU

VAL

PRO

6

ILE

TYR

ALA

GLU

THR

LEU

ASP

GLU

ALA

LEU

7

GLU

LEU

ALA

GLU

TRP

GLN

TYR

VAL

PRO

ALA

8

GLY

PHE

GLU

VAL

THR

ARG

VAL

ARG

PRO

CYS

9

VAL

ALA

PRO

LYS

Entity 2, entity_2 84 residues - 6795.774 Da.

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

LYS

GLU

PRO

ALA

ILE

LEU

ALA

GLU

ILE

4

SER

GLY

ILE

VAL

SER

PHE

GLY

LYS

GLU

THR

5

LYS

GLY

LYS

ARG

LEU

VAL

ILE

THR

PRO

6

VAL

ASP

GLY

SER

ASP

PRO

TYR

GLU

MET

7

ILE

PRO

LYS

TRP

ARG

GLN

LEU

ASN

VAL

PHE

8

GLU

GLY

GLU

ARG

VAL

GLU

ARG

GLY

ASP

VAL

9

ILE

SER

ASP

GLY

Samples:

sample_1: Gp2-Jaw complex, [U-100% 13C; U-100% 15N], 300 mM; Gp2-Jaw complex, [U-100% 13C; U-100% 15N], 300 mM; H2O 90%; D2O 10%; NaCl 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DPX 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks