BMRB Entry 18109

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18109

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Title:
1H, 13C and 15N resonance assignment of MmpS4
Deposition date:
2011-11-29
Original release date:
2012-01-06
Authors:
Sun, Peibei; Tian, Changlin
Citation:

Citation: Sun, Peibei. "1H, 13C and 15N resonance assignment of MmpS4"  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
MmpS4, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 1773   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MmpS4: MLMRTWIPLVILVVVIVGGF TVHRIRGFFGSENRPSYSDT NLENSKPFNPKHLTYEIFGP PGTVADISYFDVNSEPQRVD GAVLPWSLHITTNDAAVMGN IVAQGNSDSIGCRITVDGKV RAERVSNEVNAYTYCLVKSA

Data sets:
Data typeCount
13C chemical shifts227
15N chemical shifts73
1H chemical shifts73

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MmpS41

Entities:

Entity 1, MmpS4 140 residues - Formula weight is not available

1   METLEUMETARGTHRTRPILEPROLEUVAL
2   ILELEUVALVALVALILEVALGLYGLYPHE
3   THRVALHISARGILEARGGLYPHEPHEGLY
4   SERGLUASNARGPROSERTYRSERASPTHR
5   ASNLEUGLUASNSERLYSPROPHEASNPRO
6   LYSHISLEUTHRTYRGLUILEPHEGLYPRO
7   PROGLYTHRVALALAASPILESERTYRPHE
8   ASPVALASNSERGLUPROGLNARGVALASP
9   GLYALAVALLEUPROTRPSERLEUHISILE
10   THRTHRASNASPALAALAVALMETGLYASN
11   ILEVALALAGLNGLYASNSERASPSERILE
12   GLYCYSARGILETHRVALASPGLYLYSVAL
13   ARGALAGLUARGVALSERASNGLUVALASN
14   ALATYRTHRTYRCYSLEUVALLYSSERALA

Samples:

sample_1: MmpS4, [U-99% 13C; U-99% 15N], 0.5 mM; Na2HPO4/NaH2PO4 50 mM; DTT 2 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 700 MHz

Related Database Links:

BMRB 18602
PDB
DBJ BAH24756 BAL64310 BAQ04318
EMBL CAL70475 CCC25525 CCC42789 CCC63051 CCE35989
GB AAK44690 ABQ72177 ABR04800 ACT23482 AEB02585
REF NP_214965 NP_854122 WP_003402272 WP_015456133 WP_016719779
SP P0A5K3 P9WJS8 P9WJS9
AlphaFold P0A5K3 P9WJS8 P9WJS9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks