BMRB Entry 18090

Title:
Solution structure of the yeast Sti1 DP1 domain   PubMed: 22227520
Deposition date:
2011-11-17
Original release date:
2012-01-11
Authors:
Schmid, Andreas; Lagleder, Stephan; Graewert, Melissa; Roehl, Alina; Hagn, Franz; Wandinger, Sebastian; Cox, Marc; Demmer, Oliver; Richter, Klaus; Groll, Michael; Kessler, Horst; Buchner, Johannes
Citation:

Citation: Schmid, Andreas; Lagleder, Stephan; Grawert, Melissa Ann; Rohl, Alina; Hagn, Franz; Wandinger, Sebastian; Cox, Marc; Demmer, Oliver; Richter, Klaus; Groll, Michael; Kessler, Horst; Buchner, Johannes. "The architecture of functional modules in the Hsp90 co-chaperone Sti1/Hop."  EMBO J. 31, 1506-1517 (2012).

Assembly members:

Assembly members:
DP1, polymer, 71 residues, 7935.323 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET28A

Experimental source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET28A

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts321
15N chemical shifts76
1H chemical shifts544

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DP11

Entities:

Entity 1, DP1 71 residues - 7935.323 Da.

1   GLNPROASPLEUGLYLEUTHRGLNLEUPHE
2   ALAASPPROASNLEUILEGLUASNLEULYS
3   LYSASNPROLYSTHRSERGLUMETMETLYS
4   ASPPROGLNLEUVALALALYSLEUILEGLY
5   TYRLYSGLNASNPROGLNALAILEGLYGLN
6   ASPLEUPHETHRASPPROARGLEUMETTHR
7   ILEMETALATHRLEUMETGLYVALASPLEU
8   ASN

Samples:

sample_1: DP1, [U-99% 13C; U-99% 15N], 0.5 – 3 mM; potassium chloride 50 mM; potassium phosphate 50 mM; sodium azide 0.2 w/v; H2O 95%; D2O 5%

sample_2: DP1 0.5 mM; potassium chloride 50 mM; potassium phosphate 50 mM; sodium azide 0.2 w/v; H2O 95%; D2O 5%

sample_3: DP1, [U-99% 15N], 0.5 mM; potassium chloride 50 mM; potassium phosphate 50 mM; sodium azide 0.2 w/v; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.35 M; pH: 7.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1
3D CNH NOESYsample_1isotropicsample_conditions_1
3D CCH NOESYsample_1isotropicsample_conditions_1
3D NNH NOESYsample_3isotropicsample_conditions_1
2D HBCBCGCDHDsample_1isotropicsample_conditions_1
2D HBCBCGCDCEHEsample_1isotropicsample_conditions_1
2D 1H-15N HSQC-IPAPsample_3anisotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis

TOPSPIN, Bruker Biospin - collection, processing

ProcheckNMR, Laskowski, MacArthur, Smith, Jones, Hutchinson, Morris, Moss and Tho - data analysis

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker DMX 750 MHz
  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ GAA26351
EMBL CAA60743 CAA99217
GB AAA35121 AHY77333 AJP41564 AJT70985 AJT71475
REF NP_014670
SP P15705
TPG DAA10809
AlphaFold P15705

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks