BMRB Entry 18025
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR18025
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Citation: Matta-Camacho, Edna; Kozlov, Guennadi; Gehring, Kalle. "1H and 15N Assigned Chemical Shifts for hUBR2 ubr-box" .
Assembly members:
ubr2-box, polymer, 75 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6P1
Entity Sequences (FASTA):
ubr2-box: GPLGSLCGRVFKVGEPTYSC
RDCAVDPTCVLCMECFLGSI
HRDHRYRMTTSGGGGFCDCG
DTEAWKEGPYCQKHE
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 64 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hUBR2 ubr-box | 1 |
Entities:
Entity 1, hUBR2 ubr-box 75 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLY | SER | LEU | CYS | GLY | ARG | VAL | ||||
| 2 | PHE | LYS | VAL | GLY | GLU | PRO | THR | TYR | SER | CYS | ||||
| 3 | ARG | ASP | CYS | ALA | VAL | ASP | PRO | THR | CYS | VAL | ||||
| 4 | LEU | CYS | MET | GLU | CYS | PHE | LEU | GLY | SER | ILE | ||||
| 5 | HIS | ARG | ASP | HIS | ARG | TYR | ARG | MET | THR | THR | ||||
| 6 | SER | GLY | GLY | GLY | GLY | PHE | CYS | ASP | CYS | GLY | ||||
| 7 | ASP | THR | GLU | ALA | TRP | LYS | GLU | GLY | PRO | TYR | ||||
| 8 | CYS | GLN | LYS | HIS | GLU |
Samples:
sample_1: ubr2-box, [U-15N], 1 mM; zinc 10 uM; sodium chloride 10 mM; TRIS 20 mM; beta-mercaptoethanol 2 mM
sample_conditions_1: ionic strength: 0.02 M; pH: 7.6; pressure: 1 atm; temperature: 301 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, BRUKER - collection, processing
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAE25301 BAE27917 BAJ06628 |
| GB | AAH24217 AAH64512 AAH75642 AAI56046 AAL32101 |
| REF | NP_001170845 NP_001171542 NP_001171730 NP_001179567 NP_056070 |
| SP | Q6WKZ8 Q8IWV8 |
| TPG | DAA16585 |
| AlphaFold | Q8IWV8 Q6WKZ8 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks