BMRB Entry 17998

Name: Resonance assignments of the PPIase domain of FKBP42 from Arabidopsis thaliana
Published: 2012-10-01

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17998

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance assignments of the PPIase domain of FKBP42 from Arabidopsis thaliana

Deposition date:

2011-10-14

Original release date:

2012-10-01

Authors:

Burgardt, Noelia; Linnert, Miriam; Weiwad, Matthias; Geisler, Markus; Luecke, Christian

Citation:

Citation: Burgardt, Noelia; Linnert, Miriam; Weiwad, Matthias; Geisler, Markus; Luecke, Christian. "NMR assignments of the FKBP-type PPIase domain of FKBP42 from Arabidopsis thaliana" Biomol. NMR Assignments 6, 185-188 (2012).
PubMed: 22198817

Assembly members:

Assembly members:
FKBP42, polymer, 180 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FKBP42: MDESLEHQTQTHDQESEIVT EGSAVVHSEPSQEGNVPPKV DSEAEVLDEKVSKQIIKEGH GSKPSKYSTCFLHYRAWTKN SQHKFEDTWHEQQPIELVLG KEKKELAGLAIGVASMKSGE RALVHVGWELAYGKEGNFSF PNVPPMADLLYEVEVIGFDE TKEGKARSDMTVEERIGAAD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	682
15N chemical shifts	170
1H chemical shifts	170

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FKBP42	1

Entities:

Entity 1, FKBP42 180 residues - Formula weight is not available

1	MET	ASP	GLU	SER	LEU	GLU	HIS	GLN	THR	GLN
2	THR	HIS	ASP	GLN	GLU	SER	GLU	ILE	VAL	THR
3	GLU	GLY	SER	ALA	VAL	VAL	HIS	SER	GLU	PRO
4	SER	GLN	GLU	GLY	ASN	VAL	PRO	PRO	LYS	VAL
5	ASP	SER	GLU	ALA	GLU	VAL	LEU	ASP	GLU	LYS
6	VAL	SER	LYS	GLN	ILE	ILE	LYS	GLU	GLY	HIS
7	GLY	SER	LYS	PRO	SER	LYS	TYR	SER	THR	CYS
8	PHE	LEU	HIS	TYR	ARG	ALA	TRP	THR	LYS	ASN
9	SER	GLN	HIS	LYS	PHE	GLU	ASP	THR	TRP	HIS
10	GLU	GLN	GLN	PRO	ILE	GLU	LEU	VAL	LEU	GLY
11	LYS	GLU	LYS	LYS	GLU	LEU	ALA	GLY	LEU	ALA
12	ILE	GLY	VAL	ALA	SER	MET	LYS	SER	GLY	GLU
13	ARG	ALA	LEU	VAL	HIS	VAL	GLY	TRP	GLU	LEU
14	ALA	TYR	GLY	LYS	GLU	GLY	ASN	PHE	SER	PHE
15	PRO	ASN	VAL	PRO	PRO	MET	ALA	ASP	LEU	LEU
16	TYR	GLU	VAL	GLU	VAL	ILE	GLY	PHE	ASP	GLU
17	THR	LYS	GLU	GLY	LYS	ALA	ARG	SER	ASP	MET
18	THR	VAL	GLU	GLU	ARG	ILE	GLY	ALA	ALA	ASP

Samples:

sample_1: FKBP42, [U-15N], 0.7 mM; sodium phosphate 10 mM; sodium azide 0.05 mM

sample_2: FKBP42, [U-13C; U-15N], 2 mM; sodium phosphate 10 mM; sodium azide 0.05 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N TROSY	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HN(CA)CO	sample_2	isotropic	sample_conditions_1
3D CC(CO)NH	sample_2	isotropic	sample_conditions_1
3D H(CC)(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection, processing

SPARKY, Goddard - data analysis

NMR spectrometers:

Bruker DRX 500 MHz
Bruker Avance 800 MHz

PDB	2F4E 2IF4
DBJ	BAB02359
EMBL	CAC00654
GB	AAM13008 AAN65079 AEE76533
REF	NP_188801
SP	Q9LDC0
AlphaFold	Q9LDC0

1	MET	ASP	GLU	SER	LEU	GLU	HIS	GLN	THR	GLN
2	THR	HIS	ASP	GLN	GLU	SER	GLU	ILE	VAL	THR
3	GLU	GLY	SER	ALA	VAL	VAL	HIS	SER	GLU	PRO
4	SER	GLN	GLU	GLY	ASN	VAL	PRO	PRO	LYS	VAL
5	ASP	SER	GLU	ALA	GLU	VAL	LEU	ASP	GLU	LYS
6	VAL	SER	LYS	GLN	ILE	ILE	LYS	GLU	GLY	HIS
7	GLY	SER	LYS	PRO	SER	LYS	TYR	SER	THR	CYS
8	PHE	LEU	HIS	TYR	ARG	ALA	TRP	THR	LYS	ASN
9	SER	GLN	HIS	LYS	PHE	GLU	ASP	THR	TRP	HIS
10	GLU	GLN	GLN	PRO	ILE	GLU	LEU	VAL	LEU	GLY
11	LYS	GLU	LYS	LYS	GLU	LEU	ALA	GLY	LEU	ALA
12	ILE	GLY	VAL	ALA	SER	MET	LYS	SER	GLY	GLU
13	ARG	ALA	LEU	VAL	HIS	VAL	GLY	TRP	GLU	LEU
14	ALA	TYR	GLY	LYS	GLU	GLY	ASN	PHE	SER	PHE
15	PRO	ASN	VAL	PRO	PRO	MET	ALA	ASP	LEU	LEU
16	TYR	GLU	VAL	GLU	VAL	ILE	GLY	PHE	ASP	GLU
17	THR	LYS	GLU	GLY	LYS	ALA	ARG	SER	ASP	MET
18	THR	VAL	GLU	GLU	ARG	ILE	GLY	ALA	ALA	ASP

1	MET	ASP	GLU	SER	LEU	GLU	HIS	GLN	THR	GLN
2	THR	HIS	ASP	GLN	GLU	SER	GLU	ILE	VAL	THR
3	GLU	GLY	SER	ALA	VAL	VAL	HIS	SER	GLU	PRO
4	SER	GLN	GLU	GLY	ASN	VAL	PRO	PRO	LYS	VAL
5	ASP	SER	GLU	ALA	GLU	VAL	LEU	ASP	GLU	LYS
6	VAL	SER	LYS	GLN	ILE	ILE	LYS	GLU	GLY	HIS
7	GLY	SER	LYS	PRO	SER	LYS	TYR	SER	THR	CYS
8	PHE	LEU	HIS	TYR	ARG	ALA	TRP	THR	LYS	ASN
9	SER	GLN	HIS	LYS	PHE	GLU	ASP	THR	TRP	HIS
10	GLU	GLN	GLN	PRO	ILE	GLU	LEU	VAL	LEU	GLY
11	LYS	GLU	LYS	LYS	GLU	LEU	ALA	GLY	LEU	ALA
12	ILE	GLY	VAL	ALA	SER	MET	LYS	SER	GLY	GLU
13	ARG	ALA	LEU	VAL	HIS	VAL	GLY	TRP	GLU	LEU
14	ALA	TYR	GLY	LYS	GLU	GLY	ASN	PHE	SER	PHE
15	PRO	ASN	VAL	PRO	PRO	MET	ALA	ASP	LEU	LEU
16	TYR	GLU	VAL	GLU	VAL	ILE	GLY	PHE	ASP	GLU
17	THR	LYS	GLU	GLY	LYS	ALA	ARG	SER	ASP	MET
18	THR	VAL	GLU	GLU	ARG	ILE	GLY	ALA	ALA	ASP