BMRB Entry 17989

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PDB ID: 2lw1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17989
MolProbity Validation Chart

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Title:
NMR resonance assignment of the UUP protein C-terminal domain
Deposition date:
2011-10-11
Original release date:
2012-01-31
Authors:
Carlier, Ludovic; Haase, Sander; Lequin, Olivier
Citation:

Citation: Carlier, Ludovic; Haase, A. Sander; Burgos Zepeda, Monica; Dassa, Elie; Lequin, Olivier. "Secondary structure and NMR resonance assignments of the C-terminal DNA-binding domain of Uup protein."  Biomol. NMR Assignments 6, 197-200 (2012).
PubMed: 22287065

Assembly members:

Assembly members:
UUP, polymer, 112 residues, 12563 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UUP: GSHMGQQEQYVALKQPAVKK TEEAAAAKAETVKRSSSKLS YKLQRELEQLPQLLEDLEAK LEALQTQVADASFFSQPHEQ TQKVLADMAAAEQELEQAFE RWEYLEALKNGG

Data sets:
Data typeCount
13C chemical shifts478
15N chemical shifts113
1H chemical shifts771

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1UUP protein C-terminal domain1

Entities:

Entity 1, UUP protein C-terminal domain 112 residues - 12563 Da.

Residues 1-4 represent a non-native affinity tag. Gly-5 corresponds to Gly-528 in the native UUP protein sequence.

1   GLYSERHISMETGLYGLNGLNGLUGLNTYR
2   VALALALEULYSGLNPROALAVALLYSLYS
3   THRGLUGLUALAALAALAALALYSALAGLU
4   THRVALLYSARGSERSERSERLYSLEUSER
5   TYRLYSLEUGLNARGGLULEUGLUGLNLEU
6   PROGLNLEULEUGLUASPLEUGLUALALYS
7   LEUGLUALALEUGLNTHRGLNVALALAASP
8   ALASERPHEPHESERGLNPROHISGLUGLN
9   THRGLNLYSVALLEUALAASPMETALAALA
10   ALAGLUGLNGLULEUGLUGLNALAPHEGLU
11   ARGTRPGLUTYRLEUGLUALALEULYSASN
12   GLYGLY

Related Database Links:

PDB 2LW1
DBJ BAA35707 BAB34456 BAG76534 BAI24392 BAI29842
EMBL CAA70589 CAP75412 CAQ31477 CAQ88615 CAQ97854
GB AAC74035 AAG55435 AAN42579 AAN79553 AAP16463
REF NP_309060 NP_415469 NP_706872 WP_000053029 WP_000053031
SP P43672
AlphaFold P43672

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