BMRB Entry 17978

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17978
MolProbity Validation Chart

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Title:
Backbone and sidechain 1H, 13C and 15N chemical shift assignments of the hydrophobin MPG1 from the rice blast fungus Magnaporthe oryzae
Deposition date:
2011-10-06
Original release date:
2012-06-05
Authors:
Rey, Anthony; Kwan, Ann; Sunde, Margaret
Citation:

Citation: Rey, Anthony; Hocher, Antoine; Kwan, Ann; Sunde, Margaret. "Backbone and sidechain (1)H, (13)C and (15)N chemical shift assignments of the hydrophobin MPG1 from the rice blast fungus Magnaporthe oryzae."  Biomol. NMR Assignments 7, 109-112 (2013).
PubMed: 22610311

Assembly members:

Assembly members:
MPG1, polymer, 95 residues, 9863.39 Da.

Natural source:

Natural source:   Common Name: rice blast fungus   Taxonomy ID: 318829   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Magnaporthe oryzae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHUE-MPG1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MPG1: SAIPAPGEGPSVSMAQQKCG AEKVVSCCNSKELKNSKSGA EIPIDVLSGECKNIPINILT INQLIPINNFCSDTVSCCSG EQIGLVNIQCTPILS

Data sets:
Data typeCount
13C chemical shifts382
15N chemical shifts103
1H chemical shifts645

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MPG11

Entities:

Entity 1, MPG1 95 residues - 9863.39 Da.

Residue 1 (S1) is an artifact from cloning.

1   SERALAILEPROALAPROGLYGLUGLYPRO
2   SERVALSERMETALAGLNGLNLYSCYSGLY
3   ALAGLULYSVALVALSERCYSCYSASNSER
4   LYSGLULEULYSASNSERLYSSERGLYALA
5   GLUILEPROILEASPVALLEUSERGLYGLU
6   CYSLYSASNILEPROILEASNILELEUTHR
7   ILEASNGLNLEUILEPROILEASNASNPHE
8   CYSSERASPTHRVALSERCYSCYSSERGLY
9   GLUGLNILEGLYLEUVALASNILEGLNCYS
10   THRPROILELEUSER

Samples:

15N-MPG1: MPG1, [U-98% 15N], 300-400 uM; sodium phosphate 20 mM; D2O, [U-2H], 5%; H2O 95%; DSS 0.1 mM

15N13C-MPG1: MPG1, [U-98% 13C; U-98% 15N], 300-400 uM; sodium phosphate 20 mM; chloramphenicol 20 uM; complete EDTA protease cocktail tablet 0.63 mg/mL; H2O 95%; D2O, [U-99% 2H], 5%; DSS 0.1 mM

15N13C-MPG1_D2O: MPG1, [U-98% 13C; U-98% 15N], 300-400 uM; sodium phosphate 20 mM; chloramphenicol 20 uM; complete EDTA protease cocktail tablet 0.63 mg/mL; D2O, [U-99% 2H], 100%; DSS 0.1 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N13C-MPG1isotropicsample_conditions_1
2D 1H-13C HSQC15N13C-MPG1_D2Oisotropicsample_conditions_1
3D HNCACB15N13C-MPG1isotropicsample_conditions_1
3D HN(CA)CO15N13C-MPG1isotropicsample_conditions_1
3D CC(CO)NH15N13C-MPG1isotropicsample_conditions_1
3D HC(CO)NH15N13C-MPG1isotropicsample_conditions_1
3D CBCA(CO)NH15N13C-MPG1isotropicsample_conditions_1
3D 1H-15N NOESY15N13C-MPG1isotropicsample_conditions_1
3D CCH-TOCSY15N13C-MPG1_D2Oisotropicsample_conditions_1
3D HNCO15N13C-MPG1isotropicsample_conditions_1
3D HCAN15N13C-MPG1_D2Oisotropicsample_conditions_1
3D HCA(CO)N15N13C-MPG1_D2Oisotropicsample_conditions_1
3D 1H-13C NOESY aliphatic15N13C-MPG1_D2Oisotropicsample_conditions_1
3D HCCH-TOCSY15N13C-MPG1_D2Oisotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

SPARKY v3.1, Goddard - chemical shift assignment, peak picking

TALOS vTALOS+, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

GB AAA20128 AAX53646 AAX53647 AAX53648 AAX53649
REF XP_003720513
SP P52751
AlphaFold P52751

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks