BMRB Entry 17936

Name: N/A
Published: 2012-01-09

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PDB ID: 2ljh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17936
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

N/A

Deposition date:

2011-09-13

Original release date:

2012-01-09

Authors:

Barraud, Pierre; Allain, Frederic

Citation:

Citation: Barraud, Pierre; Heale, Bret; Allain, Mary. "Solution structure of the N-terminal dsRBD of Drosophila ADAR and interaction studies with RNA." Biochimie 94, 1499-1509 (2012).
PubMed: 22210494

Assembly members:

Assembly members:
dADAR-dsRBD1, polymer, 93 residues, 10262.124 Da.

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dADAR-dsRBD1: SDPKKKMCKERIPQPKNTVA MLNELRHGLIYKLESQTGPV HAPLFTISVEVDGQKYLGQG RSKKVARIEAAATALRSFIQ FKDGAVLSPLKPA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	263
15N chemical shifts	75
1H chemical shifts	559

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	dADAR-dsRBD1	1

Entities:

Entity 1, dADAR-dsRBD1 93 residues - 10262.124 Da.

1

SER

ASP

PRO

LYS

MET

CYS

LYS

GLU

2

ARG

ILE

PRO

GLN

PRO

LYS

ASN

THR

VAL

ALA

3

MET

LEU

ASN

GLU

LEU

ARG

HIS

GLY

LEU

ILE

4

TYR

LYS

LEU

GLU

SER

GLN

THR

GLY

PRO

VAL

5

HIS

ALA

PRO

LEU

PHE

THR

ILE

SER

VAL

GLU

6

VAL

ASP

GLY

GLN

LYS

TYR

LEU

GLY

GLN

GLY

7

ARG

SER

LYS

VAL

ALA

ARG

ILE

GLU

ALA

8

ALA

THR

ALA

LEU

ARG

SER

PHE

ILE

GLN

9

PHE

LYS

ASP

GLY

ALA

VAL

LEU

SER

PRO

LEU

10

LYS

PRO

ALA

Samples:

sample_15N: dADAR-dsRBD1, [U-15N], 0.8 – 1.2 mM; H2O 90%; D2O 10%

sample_13C15N: dADAR-dsRBD1, [U-13C; U-15N], 0.8 – 1.2 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 25 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_15N	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_13C15N	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_13C15N	isotropic	sample_conditions_1
3D HNCA	sample_13C15N	isotropic	sample_conditions_1
3D HNCACB	sample_13C15N	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_13C15N	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_13C15N	isotropic	sample_conditions_1
3D H(CCO)NH	sample_13C15N	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_15N	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_13C15N	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_13C15N	isotropic	sample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

CNS v2.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 600 MHz
Bruker Avance 700 MHz
Bruker Avance 900 MHz

PDB	2LJH
EMBL	CAA22774
GB	AAF45665 AAF63702 AAF63703 AAK26850 AAN09057
REF	NP_001033821 NP_001245469 NP_001245470 NP_001245471 NP_001245472
SP	Q9NII1
AlphaFold	Q9NII1