BMRB Entry 17921

Name: Partial 1H, 15N Chemical Shift Assignments of a GAAA Tetraloop Receptor Variant.
Published: 2012-02-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17921

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Partial 1H, 15N Chemical Shift Assignments of a GAAA Tetraloop Receptor Variant.

Deposition date:

2011-09-06

Original release date:

2012-02-08

Authors:

Vander Muelen, Kirk; Davis, Jared; Clos, Lawrence; Butcher, Samuel

Citation:

Citation: Vander Muelen, Kirk; Davis, Jared; Clos, Lawrence; Butcher, Samuel. "Partial 1H, 15N Chemical Shift Assignments of a GAAA Tetraloop Receptor Variant." The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
TECTO47, polymer, 47 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TECTO47: GGAGGAUAUGGAAGAACCGG GGUGACUUGGUUCUUCCUAA GUCCUCC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	22
1H chemical shifts	32

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GAAA tetraloop monomer	1

Entities:

Entity 1, GAAA tetraloop monomer 47 residues - Formula weight is not available

1

G

A

G

A

U

A

U

G

2

G

A

G

A

C

G

3

G

U

G

A

C

U

G

4

U

C

U

C

U

A

5

G

U

C

U

C

Samples:

sample_unlabeled: TECTO47 1 mM; DTT 10 uM; potassium phosphate 15 mM; H2O 90%; D2O 10%

sample_labeled: TECTO47, [U-100% 13C; U-100% 15N], 1 mM; DTT 10 uM; potassium phosphate 15 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 15 mM; pH: 7.0; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_labeled	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_unlabeled	isotropic	sample_conditions_1

Software:

xwinnmr v3.5, Bruker Biospin - collection, processing

SPARKY v3.114, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 600 MHz
Bruker DMX 750 MHz
Varian INOVA 900 MHz