BMRB Entry 17917

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17917

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Title:
Backbone chemical shift assignments for the C-terminal domain of yeast Tfg1.
Deposition date:
2011-09-06
Original release date:
2011-12-14
Authors:
Kilpatrick, Adina; Koharudin, Leonardus; Gronenborn, Angela
Citation:

Citation: Kilpatrick, Adina; Koharudin, Leonardus; Calero, Guillermo; Gronenborn, Angela. "Structural and binding studies of the C-terminal domains of yeast TFIIF subunits Tfg1 and Tfg2."  Proteins ., .-. (2011).
PubMed: 22095626

Assembly members:

Assembly members:
Tfg1_C-terminal_domain, polymer, 62 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-18

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Tfg1_C-terminal_domain: MASTITEKDIIEAIGDGKVN IKEFGKFIRRKYPGAENKKL MFAIVKKLCRKVGNDHMELK KE

Data sets:
Data typeCount
13C chemical shifts179
15N chemical shifts61
1H chemical shifts124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tfg11

Entities:

Entity 1, Tfg1 62 residues - Formula weight is not available

1   METALASERTHRILETHRGLULYSASPILE
2   ILEGLUALAILEGLYASPGLYLYSVALASN
3   ILELYSGLUPHEGLYLYSPHEILEARGARG
4   LYSTYRPROGLYALAGLUASNLYSLYSLEU
5   METPHEALAILEVALLYSLYSLEUCYSARG
6   LYSVALGLYASNASPHISMETGLULEULYS
7   LYSGLU

Samples:

sample_1: Tfg1 C-terminal domain, [U-98% 13C; U-98% 15N], 1 – 1.5 mM; sodium phosphate 20 mM; sodium chloride 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz

Related Database Links:

DBJ GAA23558
EMBL CAA67530 CAA97212 CAY79945
GB AAA61640 AAA68478 AHY79527 AJP38957 AJR76277
REF NP_011702
SP P41895
TPG DAA08280
AlphaFold P41895

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks