BMRB Entry 17909

Name: Resonance assignments of human Steroidogenic acute regulatory-related lipid transfer domain protein 5
Published: 2011-10-03

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17909

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance assignments of human Steroidogenic acute regulatory-related lipid transfer domain protein 5

Deposition date:

2011-09-01

Original release date:

2011-10-03

Authors:

Lorin, Aurelien; Letourneau, Danny; Lefebvre, Andree; LeHoux, Jean-Guy; Lavigne, Pierre

Citation:

Citation: Lorin, Aurelien; Letourneau, Danny; Lefebvre, Andree; LeHoux, Jean-Guy; Lavigne, Pierre. "(1)H, (13)C, and (15)N backbone chemical shift assignments of StAR-related lipid transfer domain protein 5 (STARD5)." Biomol. NMR Assignments 7, 21-24 (2013).
PubMed: 22392336

Assembly members:

Assembly members:
StarD5, polymer, 213 residues, 23797.1169 Da.

Natural source:

Natural source: Common Name: E. coli BL21(DE3) Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
StarD5: MDPALAAQMSEAVAEKMLQY RRDTAGWKICREGNGVSVSW RPSVEFPGNLYRGEGIVYGT LEEVWDCVKPAVGGLRVKWD ENVTGFEIIQSITDTLCVSR TSTPSAAMKLISPRDFVDLV LVKRYEDGTISSNATHVEHP LCPPKPGFVRGFNHPCGCFC EPLPGEPTKTNLVTFFHTDL SGYLPQNVVDSFFPRSMTRF YANLQKAVKQFHE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	705
15N chemical shifts	190
1H chemical shifts	344

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	StarD5	1

Entities:

Entity 1, StarD5 213 residues - 23797.1169 Da.

1

MET

ASP

PRO

ALA

LEU

ALA

GLN

MET

SER

2

GLU

ALA

VAL

ALA

GLU

LYS

MET

LEU

GLN

TYR

3

ARG

ASP

THR

ALA

GLY

TRP

LYS

ILE

CYS

4

ARG

GLU

GLY

ASN

GLY

VAL

SER

VAL

SER

TRP

5

ARG

PRO

SER

VAL

GLU

PHE

PRO

GLY

ASN

LEU

6

TYR

ARG

GLY

GLU

GLY

ILE

VAL

TYR

GLY

THR

7

LEU

GLU

VAL

TRP

ASP

CYS

VAL

LYS

PRO

8

ALA

VAL

GLY

LEU

ARG

VAL

LYS

TRP

ASP

9

GLU

ASN

VAL

THR

GLY

PHE

GLU

ILE

GLN

10

SER

ILE

THR

ASP

THR

LEU

CYS

VAL

SER

ARG

11

THR

SER

THR

PRO

SER

ALA

MET

LYS

LEU

12

ILE

SER

PRO

ARG

ASP

PHE

VAL

ASP

LEU

VAL

13

LEU

VAL

LYS

ARG

TYR

GLU

ASP

GLY

THR

ILE

14

SER

ASN

ALA

THR

HIS

VAL

GLU

HIS

PRO

15

LEU

CYS

PRO

LYS

PRO

GLY

PHE

VAL

ARG

16

GLY

PHE

ASN

HIS

PRO

CYS

GLY

CYS

PHE

CYS

17

GLU

PRO

LEU

PRO

GLY

GLU

PRO

THR

LYS

THR

18

ASN

LEU

VAL

THR

PHE

HIS

THR

ASP

LEU

19

SER

GLY

TYR

LEU

PRO

GLN

ASN

VAL

ASP

20

SER

PHE

PRO

ARG

SER

MET

THR

ARG

PHE

21

TYR

ALA

ASN

LEU

GLN

LYS

ALA

VAL

LYS

GLN

22

PHE

HIS

GLU

Samples:

StarD5_1: StarD5, [U-13C; U-15N; U-2H], 1.0 mM; H2O 90%; D2O 10%

CondSet1: ionic strength: 0.050 M; pH: 7.400; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	StarD5_1	isotropic	CondSet1
Expt_4 (H[N[co[{CA\|ca[C]}]]])	StarD5_1	isotropic	CondSet1
3D HNCACB	StarD5_1	isotropic	CondSet1
3D HNCO	StarD5_1	isotropic	CondSet1
Expt_49 (H{[N]+[HA]})	StarD5_1	isotropic	CondSet1
Expt_53 (H{[N]+[HA]})	StarD5_1	isotropic	CondSet1

Software:

ANALYSIS v2.1, CCPN - chemical shift assignment

NMR spectrometers:

Varian UnityInova 600 MHz

UniProt	Q9NSY2
BMRB	18721
PDB	2R55
EMBL	CAH91861
GB	AAH04365 AAL89654 AIC52409 EAW99097 EHH27540
REF	NP_001126078 NP_001247845 NP_871629 XP_003281060 XP_003810787
SP	Q5R8P9 Q9NSY2
AlphaFold	P59094 Q5R8P9 Q9NSY2