BMRB Entry 17891

Title:
Solution Structure of KKGF   PubMed: 22593157
Deposition date:
2011-08-29
Original release date:
2012-07-09
Authors:
Montserret, Roland; Penin, Francois
Citation:

Citation: Oehler, Verena; Filipe, Ana; Montserret, Roland; da Costa, Daniel; Brown, Gaie; Penin, Francois; McLauchlan, John. "Structural analysis of hepatitis C virus core-e1 signal Peptide and requirements for cleavage of the genotype 3a signal sequence by signal Peptide peptidase."  J. Virol. 86, 7818-7828 (2012).

Assembly members:

Assembly members:
kkgf, polymer, 27 residues, 2881.567 Da.

Natural source:

Natural source:   Common Name: Hepatitis C Virus   Taxonomy ID: 11103   Superkingdom: viruses   Kingdom: not available   Genus/species: Hepacivirus Flaviviridae

Experimental source:   Production method: chemical synthesis

Experimental source:

Natural source:   Common Name: Hepatitis C Virus   Taxonomy ID: 11103   Superkingdom: viruses   Kingdom: not available   Genus/species: Hepacivirus Flaviviridae

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
kkgf: KKGFPFSIFLLALLSCITVP VSAAQVK

Data sets:
Data typeCount
13C chemical shifts75
1H chemical shifts164

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1kkgf1

Entities:

Entity 1, kkgf 27 residues - 2881.567 Da.

1   LYSLYSGLYPHEPROPHESERILEPHELEU
2   LEUALALEULEUSERCYSILETHRVALPRO
3   VALSERALAALAGLNVALLYS

Samples:

sample_1: kkgf 2 mM; DSS 0.01 mM; H2O 50%; Trifluoroethanol D2OH 50%

sample_conditions_1: ionic strength: 0 M; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

Molmol, Koradi, Billeter and Wuthrich - structure analysis

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

BMRB 16601
PDB
DBJ BAB32872 BAF34893 BAF95986
GB ABN54499 ABN54513 ABN54545 ABN54546 ABN54549
SP Q99IB8