BMRB Entry 17833

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17833
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
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All files associated with the entry

Title:
Skint1 IgV
Deposition date:
2011-08-05
Original release date:
2016-02-29
Authors:
Salim, Mahboob; Knowles, Timothy; Willcox, Carrie; Mohammed, Fiyaz; Woodard, Martin; Overduin, Michael; Hayday, Adrian; Willcox, Benjamin
Citation:

Citation: Salim, Mahboob; Knowles, Timothy; Willcox, Carrie; Mohammed, Fiyaz; Woodard, Martin; Overduin, Michael; Hayday, Adrian; Willcox, Benjamin. "Characterisation of a putative receptor binding surface on Skint-1, a critical determinant of dendritic epidermal T cell selection"  J. Biol. Chem. ., .-. (2016).

Assembly members:

Assembly members:
Skint1_IgV, polymer, 238 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET23a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Skint1_IgV: MSSEPFIVNGLEGPVLASLG GNLELSCQLSPPQQAQHMEI RWFRNLYTEPVHLYRDGKDM FGEIISKYVERTELLKDGIG EGKVTLRIFNVTVDDDGSYH CVFKDGDFYEEHITEVKITM SSEPFIVNGLEGPVLASLGG NLELSCQLSPPQQAQHMEIR WFRNLYTEPVHLYRDGKDMF GEIISKYVERTELLKDGIGE GKVTLRIFNVTVDDDGSYHC VFKDGDFYEEHITEVKIT

Data sets:
Data typeCount
13C chemical shifts424
15N chemical shifts99
1H chemical shifts641

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Skint1 IgV1

Entities:

Entity 1, Skint1 IgV 238 residues - Formula weight is not available

2 to 119 is the distal membrane Ig variable domain

1   METSERSERGLUPROPHEILEVALASNGLY
2   LEUGLUGLYPROVALLEUALASERLEUGLY
3   GLYASNLEUGLULEUSERCYSGLNLEUSER
4   PROPROGLNGLNALAGLNHISMETGLUILE
5   ARGTRPPHEARGASNLEUTYRTHRGLUPRO
6   VALHISLEUTYRARGASPGLYLYSASPMET
7   PHEGLYGLUILEILESERLYSTYRVALGLU
8   ARGTHRGLULEULEULYSASPGLYILEGLY
9   GLUGLYLYSVALTHRLEUARGILEPHEASN
10   VALTHRVALASPASPASPGLYSERTYRHIS
11   CYSVALPHELYSASPGLYASPPHETYRGLU
12   GLUHISILETHRGLUVALLYSILETHRMET
13   SERSERGLUPROPHEILEVALASNGLYLEU
14   GLUGLYPROVALLEUALASERLEUGLYGLY
15   ASNLEUGLULEUSERCYSGLNLEUSERPRO
16   PROGLNGLNALAGLNHISMETGLUILEARG
17   TRPPHEARGASNLEUTYRTHRGLUPROVAL
18   HISLEUTYRARGASPGLYLYSASPMETPHE
19   GLYGLUILEILESERLYSTYRVALGLUARG
20   THRGLULEULEULYSASPGLYILEGLYGLU
21   GLYLYSVALTHRLEUARGILEPHEASNVAL
22   THRVALASPASPASPGLYSERTYRHISCYS
23   VALPHELYSASPGLYASPPHETYRGLUGLU
24   HISILETHRGLUVALLYSILETHR

Samples:

sample_1: Skint1 IgV, [U-100% 13C; U-100% 15N], 1.4 mM; MES 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks