BMRB Entry 17829

Title:
FVIII C2 Domain
Deposition date:
2011-08-03
Original release date:
2012-03-01
Authors:
Nuzzio, Kristin; Cullinan, David; Novakovic, Valerie; Boettcher, John; Rienstra, Chad; Gilbert, Gary; Baleja, James
Citation:

Citation: Nuzzio, Kristin; Cullinan, David; Novakovic, Valerie; Boettcher, John; Rienstra, Chad; Gilbert, Gary; Baleja, James. "Backbone resonance assignments of the C2 domain of coagulation factor VIII."  Biomol. NMR Assignments 7, 31-34 (2013).
PubMed: 22392338

Assembly members:

Assembly members:
C2_Domain, polymer, 163 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BL21

Data sets:
Data typeCount
13C chemical shifts440
15N chemical shifts145
1H chemical shifts145

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C2_Domain1

Entities:

Entity 1, C2_Domain 163 residues - Formula weight is not available

1   GLYSERHISMETCYSSERMETPROLEUGLY
2   METGLUSERLYSALAILESERASPALAGLN
3   ILETHRALASERSERTYRPHETHRASNMET
4   PHEALATHRTRPSERPROSERLYSALAARG
5   LEUHISLEUGLNGLYARGSERASNALATRP
6   ARGPROGLNVALASNASNPROLYSGLUTRP
7   LEUGLNVALASPPHEGLNLYSTHRMETLYS
8   VALTHRGLYVALTHRTHRGLNGLYVALLYS
9   SERLEULEUTHRSERMETTYRVALLYSGLU
10   PHELEUILESERSERSERGLNASPGLYHIS
11   GLNTRPTHRLEUPHEPHEGLNASNGLYLYS
12   VALLYSVALPHEGLNGLYASNGLNASPSER
13   PHETHRPROVALVALASNSERLEUASPPRO
14   PROLEULEUTHRARGTYRLEUARGILEHIS
15   PROGLNSERTRPVALHISGLNILEALALEU
16   ARGMETGLUVALLEUGLYCYSGLUALAGLN
17   ASPLEUTYR

Samples:

sample_1: C2 Domain 0.8 mM; H2O 90%; D2O 10%; sodium chloride 50 mM; EDTA 50 uM; NaN3 0.01%; DSS 20 uM; imidazole 10 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks