BMRB Entry 17815

Name: Backbone 1H, 13C and 15N resonance assignments for IMP1 KH34
Published: 2012-04-23

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17815

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N resonance assignments for IMP1 KH34

Deposition date:

2011-07-29

Original release date:

2012-04-23

Authors:

Harris, Richard; Patel, Vivek; Chao, Jeffery; Girvin, Mark; Singer, Robert

Citation:

Citation: Patel, Vivek; Mitra, Somdeb; Harris, Richard; Buxbaum, Adina; Lionnet, Timothee; Brenowitz, Michael; Girvin, Mark; Levy, Matthew; Almo, Steven; Singer, Robert; Chao, Jeffery. "Spatial arrangement of an RNA zipcode identifies mRNAs under post-transcriptional control" Genes Dev. 26, 43-53 (2012).
PubMed: 22215810

Assembly members:

Assembly members:
IMP1_KH34, polymer, 172 residues, 19196 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22HT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IMP1_KH34: MEQEMVQVFIPAQAVGAIIG KKGQHIKQLSRFASASIKIA PPETPDSKVRMVIITGPPEA QFKAQGRIYGKLKEENFFGP KEEVKLETHIRVPASAAGRV IGKGGKTVNELQNLTAAEVV VPRDQTPDENDQVIVKIIGH FYASQMAQRKIRDILAQVKQ QHQKLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	428
15N chemical shifts	127
1H chemical shifts	127

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	IMP1 KH34	1

Entities:

Entity 1, IMP1 KH34 172 residues - 19196 Da.

Residues M(403) and L(567)-H(574) are cloning artifacts and C-terminal His-tag

1

MET

GLU

GLN

GLU

MET

VAL

GLN

VAL

PHE

ILE

2

PRO

ALA

GLN

ALA

VAL

GLY

ALA

ILE

GLY

3

LYS

GLY

GLN

HIS

ILE

LYS

GLN

LEU

SER

4

ARG

PHE

ALA

SER

ALA

SER

ILE

LYS

ILE

ALA

5

PRO

GLU

THR

PRO

ASP

SER

LYS

VAL

ARG

6

MET

VAL

ILE

THR

GLY

PRO

GLU

ALA

7

GLN

PHE

LYS

ALA

GLN

GLY

ARG

ILE

TYR

GLY

8

LYS

LEU

LYS

GLU

ASN

PHE

GLY

PRO

9

LYS

GLU

VAL

LYS

LEU

GLU

THR

HIS

ILE

10

ARG

VAL

PRO

ALA

SER

ALA

GLY

ARG

VAL

11

ILE

GLY

LYS

GLY

LYS

THR

VAL

ASN

GLU

12

LEU

GLN

ASN

LEU

THR

ALA

GLU

VAL

13

VAL

PRO

ARG

ASP

GLN

THR

PRO

ASP

GLU

ASN

14

ASP

GLN

VAL

ILE

VAL

LYS

ILE

GLY

HIS

15

PHE

TYR

ALA

SER

GLN

MET

ALA

GLN

ARG

LYS

16

ILE

ARG

ASP

ILE

LEU

ALA

GLN

VAL

LYS

GLN

17

GLN

HIS

GLN

LYS

LEU

GLU

HIS

18

HIS

Samples:

sample_1: IMP1 KH34, [U-13C; U-15N; U-2H], 250 uM; TRIS 25 mM; sodium chloride 300 mM; DSS 1 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 300 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CB	sample_1	isotropic	sample_conditions_1
3D HN(COCA)CB	sample_1	isotropic	sample_conditions_1

Software:

VNMRJ v2.2D, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CCPN_Analysis v2.1.2, CCPN - data analysis

NMR spectrometers:

Varian INOVA 600 MHz

PDB	3KRM
DBJ	BAG52029
GB	ABB46294 EHH57936 KFP20603 KFZ57551
REF	NP_001153895 XP_002800442 XP_003278799 XP_003414705 XP_003435309