BMRB Entry 17813

Name: NMR structure of the UHRF1 PHD domain
Published: 2011-09-01

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PDB ID: 2lgl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17813
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of the UHRF1 PHD domain

Deposition date:

2011-07-28

Original release date:

2011-09-01

Authors:

Wang, Chengkun

Citation:

Citation: Wang, Chengkun; Shen, Jie; Yang, Zhongzheng; Chen, Ping; Zhao, Bin; Hu, Wei; Lan, Wenxian; Tong, Xiaotian; Wu, Houming; Li, Guohong; Cao, Chunyang. "Structural basis for site-specific reading of unmodified R2 of histone H3 tail by UHRF1 PHD finger." Cell Res. 21, 1379-1382 (2011).
PubMed: 21808299

Assembly members:

Assembly members:
entity_1, polymer, 69 residues, 7849.913 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETDuet

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SGPSCKHCKDDVNRLCRVCA CHLCGGRQDPDKQLMCDECD MAFHIYCLDPPLSSVPSEDE WYCPECRND

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	248
15N chemical shifts	64
1H chemical shifts	376

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UHRF1 PHD domain	1
2	ZINC ION_1	2
3	ZINC ION_2	2
4	ZINC ION_3	2

Entities:

Entity 1, UHRF1 PHD domain 69 residues - 7849.913 Da.

1

SER

GLY

PRO

SER

CYS

LYS

HIS

CYS

LYS

ASP

2

ASP

VAL

ASN

ARG

LEU

CYS

ARG

VAL

CYS

ALA

3

CYS

HIS

LEU

CYS

GLY

ARG

GLN

ASP

PRO

4

ASP

LYS

GLN

LEU

MET

CYS

ASP

GLU

CYS

ASP

5

MET

ALA

PHE

HIS

ILE

TYR

CYS

LEU

ASP

PRO

6

PRO

LEU

SER

VAL

PRO

SER

GLU

ASP

GLU

7

TRP

TYR

CYS

PRO

GLU

CYS

ARG

ASN

ASP

Entity 2, ZINC ION_1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: entity_1, [U-100% 13C; U-100% 15N], 1.5 mM; sodium chloride 137 mM; potassium phosphate 10 mM; DTT 3 mM; potassium chloride 2.7 mM; sodium azide 0.1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 293.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	17808 17812
PDB	2LGG 2LGK 2LGL 3ASK 3ASL 3SHB 3SOU 3SOW 3SOX 3T6R 3ZVY 3ZVZ 4GY5
DBJ	BAF36719 BAF36720 BAF82078 BAG37156
GB	AAF28469 AAI13876 AAK55744 AAV40831 ABQ59043
REF	NP_001041666 NP_001276979 NP_001276980 NP_001276981 NP_037414
SP	Q96T88
AlphaFold	Q96T88