BMRB Entry 17782
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17782
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Citation: Mund, Markus; Neu, Ancilla; Ullmann, Janina; Neu, Ursula; Sprangers, Remco. "Structure of the LSm657 complex: an assembly intermediate of the LSm1-7 and LSm2-8 rings." J. Mol. Biol. 414, 165-176 (2011).
PubMed: 22001694
Assembly members:
lsm57, polymer, 193 residues, Formula weight is not available
Natural source: Common Name: fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 274 |
| 15N chemical shifts | 133 |
| 1H chemical shifts | 133 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | lsm57 | 1 |
Entities:
Entity 1, lsm57 193 residues - Formula weight is not available
| 1 | MET | SER | MET | THR | ILE | LEU | PRO | LEU | GLU | LEU | ||||
| 2 | ILE | ASP | LYS | CYS | ILE | GLY | SER | ASN | LEU | TRP | ||||
| 3 | VAL | ILE | MET | LYS | SER | GLU | ARG | GLU | PHE | ALA | ||||
| 4 | GLY | THR | LEU | VAL | GLY | PHE | ASP | ASP | TYR | VAL | ||||
| 5 | ASN | ILE | VAL | LEU | LYS | ASP | VAL | THR | GLU | TYR | ||||
| 6 | ASP | THR | VAL | THR | GLY | VAL | THR | GLU | LYS | HIS | ||||
| 7 | SER | GLU | MET | LEU | LEU | ASN | GLY | ASN | GLY | MET | ||||
| 8 | CYS | MET | LEU | ILE | PRO | GLY | GLY | LYS | PRO | GLU | ||||
| 9 | MET | SER | SER | LEU | GLN | LYS | ARG | PRO | GLY | PRO | ||||
| 10 | GLY | ASN | SER | SER | GLN | PRO | THR | GLU | ARG | PRO | ||||
| 11 | ARG | LYS | GLU | SER | ILE | LEU | ASP | LEU | SER | ARG | ||||
| 12 | TYR | GLN | ASP | GLN | ARG | ILE | GLN | ALA | THR | PHE | ||||
| 13 | THR | GLY | GLY | ARG | GLN | ILE | THR | GLY | ILE | LEU | ||||
| 14 | LYS | GLY | PHE | ASP | GLN | LEU | MET | ASN | LEU | VAL | ||||
| 15 | LEU | ASP | ASP | VAL | GLU | GLU | GLN | LEU | ARG | ASN | ||||
| 16 | PRO | GLU | ASP | GLY | LYS | LEU | THR | GLY | ALA | ILE | ||||
| 17 | ARG | LYS | LEU | GLY | LEU | VAL | VAL | VAL | ARG | GLY | ||||
| 18 | THR | THR | LEU | VAL | LEU | ILE | ALA | PRO | MET | ASP | ||||
| 19 | GLY | SER | GLU | GLU | ILE | PRO | ASN | PRO | PHE | VAL | ||||
| 20 | GLN | ALA | GLU |
Samples:
sample_1: lsm57, [U-100% 13C; U-100% 15N; U-80% 2H], 1 mM; H2O 95%; D2O 5%; NaPO4 pH 7.5 50 mM; NaCl 150 mM; DTT 1 mM; NaN3 0.3%
sample_conditions_1: ionic strength: 0.15 M; pH: 7.5; pressure: 1 atm; temperature: 318 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - chemical shift assignment
NMR spectrometers:
- Bruker DRX 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks