BMRB Entry 17781

Name: structure of
Published: 2011-10-25

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17781

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

structure of

Deposition date:

2011-07-14

Original release date:

2011-10-25

Authors:

Sprangers, Remco; Mund, Markus; Neu, Ancilla

Citation:

Citation: Mund, Markus; Neu, Ancilla; Ullmann, Janina; Neu, Ursula; Sprangers, Remco. "Structure of the LSm657 complex: an assembly intermediate of the LSm1-7 and LSm2-8 rings." J. Mol. Biol. 414, 165-176 (2011).
PubMed: 22001694

Assembly members:

Assembly members:
lsm, polymer, 276 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Schizosaccharomyces pombe Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet

Entity Sequences (FASTA):

Entity Sequences (FASTA):
lsm: GAMSMTILPLELIDKCIGSN LWVIMKSEREFAGTLVGFDD YVNIVLKDVTEYDTVTGVTE KHSEMLLNGNGMCMLIPGGK PEGAMDSSPNEFLNKVIGKK VLIRLSSGVDYKGILSCLDG YMNLALERTEEYVNGKKTNV YGDAFIRGNNVLYVSALDDG AMGMSSLQKRPGPGNSSQPT ERPRKESILDLSRYQDQRIQ ATFTGGRQITGILKGFDQLM NLVLDDVEEQLRNPEDGKLT GAIRKLGLVVVRGTTLVLIA PMDGSEEIPNPFVQAE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	620
15N chemical shifts	198
1H chemical shifts	198

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	lsm5	1
2	lsm6	1
3	lsm7	1

Entities:

Entity 1, lsm5 276 residues - Formula weight is not available

1

GLY

ALA

MET

SER

MET

THR

ILE

LEU

PRO

LEU

2

GLU

LEU

ILE

ASP

LYS

CYS

ILE

GLY

SER

ASN

3

LEU

TRP

VAL

ILE

MET

LYS

SER

GLU

ARG

GLU

4

PHE

ALA

GLY

THR

LEU

VAL

GLY

PHE

ASP

5

TYR

VAL

ASN

ILE

VAL

LEU

LYS

ASP

VAL

THR

6

GLU

TYR

ASP

THR

VAL

THR

GLY

VAL

THR

GLU

7

LYS

HIS

SER

GLU

MET

LEU

ASN

GLY

ASN

8

GLY

MET

CYS

MET

LEU

ILE

PRO

GLY

LYS

9

PRO

GLU

GLY

ALA

MET

ASP

SER

PRO

ASN

10

GLU

PHE

LEU

ASN

LYS

VAL

ILE

GLY

LYS

11

VAL

LEU

ILE

ARG

LEU

SER

GLY

VAL

ASP

12

TYR

LYS

GLY

ILE

LEU

SER

CYS

LEU

ASP

GLY

13

TYR

MET

ASN

LEU

ALA

LEU

GLU

ARG

THR

GLU

14

GLU

TYR

VAL

ASN

GLY

LYS

THR

ASN

VAL

15

TYR

GLY

ASP

ALA

PHE

ILE

ARG

GLY

ASN

16

VAL

LEU

TYR

VAL

SER

ALA

LEU

ASP

GLY

17

ALA

MET

GLY

MET

SER

LEU

GLN

LYS

ARG

18

PRO

GLY

PRO

GLY

ASN

SER

GLN

PRO

THR

19

GLU

ARG

PRO

ARG

LYS

GLU

SER

ILE

LEU

ASP

20

LEU

SER

ARG

TYR

GLN

ASP

GLN

ARG

ILE

GLN

21

ALA

THR

PHE

THR

GLY

ARG

GLN

ILE

THR

22

GLY

ILE

LEU

LYS

GLY

PHE

ASP

GLN

LEU

MET

23

ASN

LEU

VAL

LEU

ASP

VAL

GLU

GLN

24

LEU

ARG

ASN

PRO

GLU

ASP

GLY

LYS

LEU

THR

25

GLY

ALA

ILE

ARG

LYS

LEU

GLY

LEU

VAL

26

VAL

ARG

GLY

THR

LEU

VAL

LEU

ILE

ALA

27

PRO

MET

ASP

GLY

SER

GLU

ILE

PRO

ASN

28

PRO

PHE

VAL

GLN

ALA

GLU

Samples:

sample_1: lsm567, [U-100% 13C; U-100% 15N; U-80% 2H], 1 mM; H2O 95%; D2O 5%; NaPO4 50 mM; NaCl 150 mM; DTT 1 mM; NaN3 0.03%

sample_conditions_1: ionic strength: 250 mM; pH: 7.5; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bartels et al. - chemical shift assignment

NMR spectrometers:

Bruker DRX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks