BMRB Entry 17766

Title:
Secondary structure of subunit E (E133-222) from Saccharomyces cerevisiae V1VO ATPase
Deposition date:
2011-07-07
Original release date:
2011-08-16
Authors:
sankaranarayanan, rishikesan; grueber, gerhard
Citation:

Citation: Rishikesan, Sankaranarayanan; Gruber, Gerhard. "Structural elements of the C-terminal domain of subunit E (E133-222) from the Saccharomyces cerevisiae V1V0 ATPase determined by solution NMR spectroscopy"  J. Bioenerg. Biomembr. 43, 447-455 (2011).
PubMed: 21826517

Assembly members:

Assembly members:
E133-222, polymer, 92 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET9d

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts266
15N chemical shifts89
1H chemical shifts568

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1V-ATPase1

Entities:

Entity 1, V-ATPase 92 residues - Formula weight is not available

1   METVALLEUGLUPROLYSALAILEVALLYS
2   ALALEUGLUARGASPVALASPLEUILEGLU
3   SERMETLYSASPASPILEMETARGGLUTYR
4   GLYGLULYSALAGLNARGALAPROLEUGLU
5   GLUILEVALILESERASNASPTYRLEUASN
6   LYSASPLEUVALSERGLYGLYVALVALVAL
7   SERASNALASERASPLYSILEGLUILEASN
8   ASNTHRLEUGLUGLUARGLEULYSLEULEU
9   SERGLUGLUALALEUPROALAILEARGLEU
10   GLULEU

Samples:

sample_1: E133-222, [U-13C; U-15N], 0.5 mM; H2O 90%; D2O 10%; Na2PO4 25 mM; sodium chloride 200 mM; EDTA 5 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ GAA26638
EMBL CAA89978 CAA99654 CAY86613
GB AAA35209 AHY77605 AJP41835 AJT71258 AJT71746
REF NP_014977
SP P22203
TPG DAA11094
AlphaFold P22203

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks