BMRB Entry 17766

Name: Secondary structure of subunit E (E133-222) from Saccharomyces cerevisiae V1VO ATPase
Published: 2011-08-16

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17766

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Secondary structure of subunit E (E133-222) from Saccharomyces cerevisiae V1VO ATPase

Deposition date:

2011-07-07

Original release date:

2011-08-16

Authors:

sankaranarayanan, rishikesan; grueber, gerhard

Citation:

Citation: Rishikesan, Sankaranarayanan; Gruber, Gerhard. "Structural elements of the C-terminal domain of subunit E (E133-222) from the Saccharomyces cerevisiae V1V0 ATPase determined by solution NMR spectroscopy" J. Bioenerg. Biomembr. 43, 447-455 (2011).
PubMed: 21826517

Assembly members:

Assembly members:
E133-222, polymer, 92 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET9d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E133-222: MVLEPKAIVKALERDVDLIE SMKDDIMREYGEKAQRAPLE EIVISNDYLNKDLVSGGVVV SNASDKIEINNTLEERLKLL SEEALPAIRLEL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	266
15N chemical shifts	89
1H chemical shifts	568

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	V-ATPase	1

Entities:

Entity 1, V-ATPase 92 residues - Formula weight is not available

1

MET

VAL

LEU

GLU

PRO

LYS

ALA

ILE

VAL

LYS

2

ALA

LEU

GLU

ARG

ASP

VAL

ASP

LEU

ILE

GLU

3

SER

MET

LYS

ASP

ILE

MET

ARG

GLU

TYR

4

GLY

GLU

LYS

ALA

GLN

ARG

ALA

PRO

LEU

GLU

5

GLU

ILE

VAL

ILE

SER

ASN

ASP

TYR

LEU

ASN

6

LYS

ASP

LEU

VAL

SER

GLY

VAL

7

SER

ASN

ALA

SER

ASP

LYS

ILE

GLU

ILE

ASN

8

ASN

THR

LEU

GLU

ARG

LEU

LYS

LEU

9

SER

GLU

ALA

LEU

PRO

ALA

ILE

ARG

LEU

10

GLU

LEU

Samples:

sample_1: E133-222, [U-13C; U-15N], 0.5 mM; H2O 90%; D2O 10%; Na2PO4 25 mM; sodium chloride 200 mM; EDTA 5 mM

sample_conditions_1: ionic strength: 200 mM; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

Bruker Avance 600 MHz

PDB	3J9T 3J9U 3J9V 4DL0 4EFA
DBJ	GAA26638
EMBL	CAA89978 CAA99654 CAY86613
GB	AAA35209 AHY77605 AJP41835 AJT71258 AJT71746
REF	NP_014977
SP	P22203
TPG	DAA11094
AlphaFold	P22203