BMRB Entry 17755

Name: 1H, 13C, and 15N resonance assignments of human regenerating family I-alpha protein (Reg I-alpha)
Published: 2011-10-12

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17755

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N resonance assignments of human regenerating family I-alpha protein (Reg I-alpha)

Deposition date:

2011-07-01

Original release date:

2011-10-12

Authors:

Ho, Meng-Ru; Chen, Chinpan

Citation:

Citation: Ho, Meng-Ru; Chen, Chinpan. "1H, 13C, and 15N resonance assignments for human regenerating family I protein." Biomol. NMR Assignments 6, 135-137 (2012).
PubMed: 21918816

Assembly members:

Assembly members:
Human_Reg_I-alpha, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Human_Reg_I-alpha: SCPEGTNAYRSYCYYFNEDR ETWVDADLYCQNMNSGNLVS VLTQAEGAFVASLIKESGTD DFNVWIGLHDPKKNRRWHWS SGSLVSYKSWGIGAPSSVNP GYCVSLTSSTGFQKWKDVPC EDKFSFVCKFKN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	552
15N chemical shifts	144
1H chemical shifts	837

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Reg I-alpha monomer	1

Entities:

Entity 1, Reg I-alpha monomer 132 residues - Formula weight is not available

1

SER

CYS

PRO

GLU

GLY

THR

ASN

ALA

TYR

ARG

2

SER

TYR

CYS

TYR

PHE

ASN

GLU

ASP

ARG

3

GLU

THR

TRP

VAL

ASP

ALA

ASP

LEU

TYR

CYS

4

GLN

ASN

MET

ASN

SER

GLY

ASN

LEU

VAL

SER

5

VAL

LEU

THR

GLN

ALA

GLU

GLY

ALA

PHE

VAL

6

ALA

SER

LEU

ILE

LYS

GLU

SER

GLY

THR

ASP

7

ASP

PHE

ASN

VAL

TRP

ILE

GLY

LEU

HIS

ASP

8

PRO

LYS

ASN

ARG

TRP

HIS

TRP

SER

9

SER

GLY

SER

LEU

VAL

SER

TYR

LYS

SER

TRP

10

GLY

ILE

GLY

ALA

PRO

SER

VAL

ASN

PRO

11

GLY

TYR

CYS

VAL

SER

LEU

THR

SER

THR

12

GLY

PHE

GLN

LYS

TRP

LYS

ASP

VAL

PRO

CYS

13

GLU

ASP

LYS

PHE

SER

PHE

VAL

CYS

LYS

PHE

14

LYS

ASN

Samples:

sample_1: Reg I-alpha, [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 – 1.0 mM; NaH2PO4 20 mM; sodium chloride 50 mM; potassium chloride 50 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 50 mM; pH: 4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

PDB	1LIT 1QDD
DBJ	BAF84670 BAI45866
GB	AAA36558 AAA36559 AAA60546 AAD51330 AAH05350
PRF	1617122A
REF	NP_002900 XP_001163554 XP_002811895 XP_003282186 XP_003816658
SP	P05451
AlphaFold	P05451