BMRB Entry 17731

Title:
Structural Plasticity of Paneth cell alpha-Defensins: Characterization of Salt-Bridge Deficient Analogues of Mouse Cryptdin-4
Deposition date:
2011-06-24
Original release date:
2012-05-09
Authors:
Rosengren, K. Johan; Andersson, Hakan; Haugaard-Kedstrom, Linda; Bengtsson, Elina; Daly, Norelle; Craik, David
Citation:

Citation: Andersson, Hakan; Figueredo, Sharel; Haugaard-Kedstrom, Linda; Bengtsson, Elina; Daly, Norelle; Qu, Xiaoqing; Craik, David; Ouellette, Andre; Rosengren, K. Johan. "The -defensin salt-bridge induces backbone stability to facilitate folding and confer proteolytic resistance."  Amino Acids 43, 1471-1483 (2012).
PubMed: 22286872

Assembly members:

Assembly members:
entity, polymer, 32 residues, 3699.551 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GLLCYCRKGHCKRGGRVRGT CGIRFLYCCPRR

Data sets:
Data typeCount
1H chemical shifts205

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1(E15G)-Crp41

Entities:

Entity 1, (E15G)-Crp4 32 residues - 3699.551 Da.

1   GLYLEULEUCYSTYRCYSARGLYSGLYHIS
2   CYSLYSARGGLYGLYARGVALARGGLYTHR
3   CYSGLYILEARGPHELEUTYRCYSCYSPRO
4   ARGARG

Samples:

sample_1: (E15G)-Crp4 0.3 mg; H2O 90%; D2O 10%

sample_conditions_1: pH: 4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 900 MHz

Related Database Links:

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