BMRB Entry 17722

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17722

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Title:
Backbone (1H,13C,15N) chemical shifts for the two tandem Calcium Binding Domains (CBD12) of the Na+/Ca2+ exchanger in the Ca2+-bound state
Deposition date:
2011-06-20
Original release date:
2011-08-16
Authors:
Salinas, Roberto; Bruschweiler-Li, Lei; Johnson, Eric; Bruschweiler, Rafael
Citation:

Citation: Salinas, Roberto; Bruschweiler-Li, Lei; Johnson, Eric; Bruschweiler, Rafael. "Ca2+ binding alters the interdomain flexibility between the two cytoplasmic calcium-binding domains in the Na+/Ca2+ exchanger."  J. Biol. Chem. 286, 32123-32131 (2011).
PubMed: 21778234

Assembly members:

Assembly members:
CBD12, polymer, 295 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: dogs   Taxonomy ID: 9615   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Canis familiaris

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET23b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CBD12: MSHHHHHHVSKIFFEQGTYQ CLENCGTVALTIIRRGGDLT NTVFVDFRTEDGTANAGSDY EFTEGTVVFKPGETQKEIRV GIIDDDIFEEDENFLVHLSN VKVSSEASEDGILEANHVSA LACLGSPSTATVTIFDDDHA GIFTFEEPVTHVSESIGIME VKVLRTSGARGNVIVPYKTI EGTARGGGEDFEDTCGELEF QNDEIVKTISVKVIDDEEYE KNKTFFLEIGEPRLVEMSEK KGGFTITEEYDDKQPLTSKE EEERRIAEMGRPILGEHTKL EVIIEESYEFKSTVD

Data sets:
Data typeCount
13C chemical shifts373
15N chemical shifts232
1H chemical shifts232

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CBD121
2Calcium ion2

Entities:

Entity 1, CBD12 295 residues - Formula weight is not available

Residues 371-657 from the NCX1 AD splice variant expressed as an eight-residue His-tag fusion

1   METSERHISHISHISHISHISHISVALSER
2   LYSILEPHEPHEGLUGLNGLYTHRTYRGLN
3   CYSLEUGLUASNCYSGLYTHRVALALALEU
4   THRILEILEARGARGGLYGLYASPLEUTHR
5   ASNTHRVALPHEVALASPPHEARGTHRGLU
6   ASPGLYTHRALAASNALAGLYSERASPTYR
7   GLUPHETHRGLUGLYTHRVALVALPHELYS
8   PROGLYGLUTHRGLNLYSGLUILEARGVAL
9   GLYILEILEASPASPASPILEPHEGLUGLU
10   ASPGLUASNPHELEUVALHISLEUSERASN
11   VALLYSVALSERSERGLUALASERGLUASP
12   GLYILELEUGLUALAASNHISVALSERALA
13   LEUALACYSLEUGLYSERPROSERTHRALA
14   THRVALTHRILEPHEASPASPASPHISALA
15   GLYILEPHETHRPHEGLUGLUPROVALTHR
16   HISVALSERGLUSERILEGLYILEMETGLU
17   VALLYSVALLEUARGTHRSERGLYALAARG
18   GLYASNVALILEVALPROTYRLYSTHRILE
19   GLUGLYTHRALAARGGLYGLYGLYGLUASP
20   PHEGLUASPTHRCYSGLYGLULEUGLUPHE
21   GLNASNASPGLUILEVALLYSTHRILESER
22   VALLYSVALILEASPASPGLUGLUTYRGLU
23   LYSASNLYSTHRPHEPHELEUGLUILEGLY
24   GLUPROARGLEUVALGLUMETSERGLULYS
25   LYSGLYGLYPHETHRILETHRGLUGLUTYR
26   ASPASPLYSGLNPROLEUTHRSERLYSGLU
27   GLUGLUGLUARGARGILEALAGLUMETGLY
28   ARGPROILELEUGLYGLUHISTHRLYSLEU
29   GLUVALILEILEGLUGLUSERTYRGLUPHE
30   LYSSERTHRVALASP

Entity 2, Calcium ion - Ca - 40.078 Da.

1   CA

Samples:

sample_1: CBD12, [U-100% 13C; U-100% 15N; U-80% 2H], 0.5 ± 0.1 mM; H2O 90.0%; D2O 10.0%; hepes 20.0 mM; NaCl 80.0 mM; CaCl2 20.0 mM; Beta-mercaptoethanol 20.0 mM

sample_conditions_1: ionic strength: 0.12 M; pH: 7; pressure: 1 atm; temperature: 306 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1

Software:

Analysis_CCPN v2.1.5, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

SP P23685
BMRB 17721
PDB
AlphaFold P23685

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks