BMRB Entry 17717

Title:
Monomeric Mouse ApoAI(1-216)
Deposition date:
2011-06-17
Original release date:
2012-12-17
Authors:
Chen, Jianglei; Wang, Jianjun; Yang, Yunhuang
Citation:

Citation: Chen, Jianglei; Yang, Yunhuang; Wang, Jianjun. "NMR Structure of Mouse ApoAI(1-216)"  J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
entity, polymer, 216 residues, 25264.260 Da.

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30(+)

Data sets:
Data typeCount
13C chemical shifts660
15N chemical shifts230
1H chemical shifts1363

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NMR Structure of Mouse ApoAI(1-216)1

Entities:

Entity 1, NMR Structure of Mouse ApoAI(1-216) 216 residues - 25264.260 Da.

1   ASPGLUPROGLNSERGLNTRPASPLYSVAL
2   LYSASPPHEALAASNVALTYRVALASPALA
3   VALLYSASPSERGLYARGASPTYRVALSER
4   GLNPHEGLUSERSERSERLEUGLYGLNGLN
5   LEUASNLEUASNLEULEUGLUASNTRPASP
6   THRLEUGLYSERTHRVALSERGLNLEUGLN
7   GLUARGLEUGLYPROLEUTHRARGASPPHE
8   TRPASPASNLEUGLULYSGLUTHRASPTRP
9   VALARGGLNGLUMETASNLYSASPLEUGLU
10   GLUVALLYSGLNLYSVALGLNPROTYRLEU
11   ASPGLUPHEGLNLYSLYSTRPLYSGLUASP
12   VALGLULEUTYRARGGLNLYSALASERPRO
13   GLNGLYALAGLULEUGLNGLUSERALAARG
14   GLNLYSLEUGLNGLULEUGLNGLYARGLEU
15   SERPROVALALAGLUGLUPHEARGASPARG
16   METARGTHRHISVALASPSERLEUARGTHR
17   GLNLEUALAPROHISSERGLUGLNMETARG
18   GLUSERLEUALAGLNARGLEUALAGLULEU
19   LYSSERASNPROTHRLEUASNGLUTYRHIS
20   SERSERALALYSSERHISLEULYSSERLEU
21   GLYGLULYSALAARGPROALALEUGLUASP
22   LEUARGHISSERLEUMET

Samples:

13C15N_labeled: entity, [U-100% 13C; U-100% 15N], ~1.0 mM; DSS 0.03 mM; sodium azide 0.01 mM; EDTA, [U-100% 2H], 10 mM; sodium phosphate 25 mM; H2O 90%; D2O 10%

Triple_labeled: entity, [U-13C; U-15N; U-2H], ~1.0 mM; DSS 0.03 mM; sodium azide 0.01 mM; EDTA, [U-100% 2H], 10 mM; sodium phosphate 25 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC13C15N_labeledisotropicsample_conditions_1
3D HNCATriple_labeledisotropicsample_conditions_1
3D HN(CO)CATriple_labeledisotropicsample_conditions_1
3D HNCACBTriple_labeledisotropicsample_conditions_1
3D HN(COCA)CBTriple_labeledisotropicsample_conditions_1
3D 1H-15N NOESY13C15N_labeledisotropicsample_conditions_1
3D HCCH-TOCSY13C15N_labeledisotropicsample_conditions_1
3D CCC-TOCSY-NNH13C15N_labeledisotropicsample_conditions_1
4D 13C15N NOESY13C15N_labeledisotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

ProcheckNMR, Laskowski and MacArthur - refinement

NMRView, Johnson, One Moon Scientific - chemical shift assignment

PIPP, Garrett - chemical shift assignment

VNMR, Varian - collection

TALOS, Cornilescu, Delaglio and Bax - chemical shift calculation

InsightII, Accelrys Software Inc. - refinement

CSI, Johnson, One Moon Scientific - chemical shift calculation

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15091
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks