BMRB Entry 17715

Name: Itch WW domain 2
Published: 2019-08-29

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17715

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Itch WW domain 2

Deposition date:

2011-06-16

Original release date:

2019-08-29

Authors:

Barbato, Gaetano; Bellomaria, Alessia; Melino, Sonia

Citation:

Citation: Bellomaria, Alessia; Melino, Sonia; Barbato, Gaetano; Paci, Maurizio; Melino, Gerry. "Itch-E3 ligase second WW domain 1H-13C-15N assignments" Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
Itch_WW2, polymer, 49 residues, 5000 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: none

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Itch_WW2: GPLGSDRPEPLPPGWERRVD NMGRIYYVDHFTRTTTWQRP TLESVRNYE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	187
15N chemical shifts	44
1H chemical shifts	267

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	WW2	1

Entities:

Entity 1, WW2 49 residues - 5000 Da.

1

GLY

PRO

LEU

GLY

SER

ASP

ARG

PRO

GLU

PRO

2

LEU

PRO

GLY

TRP

GLU

ARG

VAL

ASP

3

ASN

MET

GLY

ARG

ILE

TYR

VAL

ASP

HIS

4

PHE

THR

ARG

THR

TRP

GLN

ARG

PRO

5

THR

LEU

GLU

SER

VAL

ARG

ASN

TYR

GLU

Samples:

sample_1: Itch WW2, [U-100% 13C; U-100% 15N], 0.6 mM; potassium chloride 50 mM; NaPi 10 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 50 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

NMRView vNMRViewJ, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific - chemical shift assignment, processing

NMR spectrometers:

Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks