BMRB Entry 17705

Name: na1
Published: 2011-10-26

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PDB ID: 2lea
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17705
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

na1

Deposition date:

2011-06-15

Original release date:

2011-10-26

Authors:

Daubner, Gerrit

Citation:

Citation: Daubner, Gerrit; Clery, Antoine; Jayne, Sandrine; Stevenin, James; Allain, Frederic H-T. "A syn-anti conformational difference allows SRSF2 to recognize guanines and cytosines equally well." EMBO J. 31, 162-174 (2012).
PubMed: 22002536

Assembly members:

Assembly members:
SRSF2_RRM, polymer, 135 residues, 11705.210 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SRSF2_RRM: MGSSHHHHHHSSGLVPRGSH MASMTGGQQMGRGSMSYGRP PPDVEGMTSLKVDNLTYRTS PDTLRRVFEKYGRVGDVYIP RDRYTKESRGFAFVRFHDKR DAEDAMDAMDGAVLDGRELR VQMARYGRPPDSHHS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	264
15N chemical shifts	96
1H chemical shifts	642

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SRSF2_RRM	1

Entities:

Entity 1, SRSF2_RRM 135 residues - 11705.210 Da.

Residues 1-34 present the HIS and T7 Tag; Residues 35-131 the RRM domain.

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

ALA

SER

MET

THR

GLY

GLN

MET

4

GLY

ARG

GLY

SER

MET

SER

TYR

GLY

ARG

PRO

5

PRO

ASP

VAL

GLU

GLY

MET

THR

SER

LEU

6

LYS

VAL

ASP

ASN

LEU

THR

TYR

ARG

THR

SER

7

PRO

ASP

THR

LEU

ARG

VAL

PHE

GLU

LYS

8

TYR

GLY

ARG

VAL

GLY

ASP

VAL

TYR

ILE

PRO

9

ARG

ASP

ARG

TYR

THR

LYS

GLU

SER

ARG

GLY

10

PHE

ALA

PHE

VAL

ARG

PHE

HIS

ASP

LYS

ARG

11

ASP

ALA

GLU

ASP

ALA

MET

ASP

ALA

MET

ASP

12

GLY

ALA

VAL

LEU

ASP

GLY

ARG

GLU

LEU

ARG

13

VAL

GLN

MET

ALA

ARG

TYR

GLY

ARG

PRO

14

ASP

SER

HIS

SER

Samples:

SRSF2_RRM_sample_1: SRSF2 RRM 1, [U-15N], 0.75 ± 0.1 mM; SRSF2 RRM 2, [U-13C; U-15N], 0.75 ± 0.1 mM; L-Arg 50 mM; L-Glu 50 mM; NaH2PO4 20 mM; H2O 90%; D2O 10%

SRSF2_RRM_D2O: SRSF2 RRM 1, [U-15N], 0.75 ± 0.1 mM; L-Arg 50 mM; L-Glu 50 mM; NaH2PO4 20 mM; D2O 100%

SRSF2_RRM_sample_conditions: ionic strength: 120 mM; pH: 5.5; pressure: 1 atm; temperature: 310.8 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	SRSF2_RRM_sample_1	isotropic	SRSF2_RRM_sample_conditions
2D 1H-13C HSQC	SRSF2_RRM_sample_1	isotropic	SRSF2_RRM_sample_conditions
2D 1H-1H TOCSY	SRSF2_RRM_D2O	isotropic	SRSF2_RRM_sample_conditions
3D HCACO	SRSF2_RRM_sample_1	isotropic	SRSF2_RRM_sample_conditions
3D HNCO	SRSF2_RRM_sample_1	isotropic	SRSF2_RRM_sample_conditions
3D HNCA	SRSF2_RRM_sample_1	isotropic	SRSF2_RRM_sample_conditions
3D HN(CO)CA	SRSF2_RRM_sample_1	isotropic	SRSF2_RRM_sample_conditions
3D CBCA(CO)NH	SRSF2_RRM_sample_1	isotropic	SRSF2_RRM_sample_conditions
3D HNCACB	SRSF2_RRM_sample_1	isotropic	SRSF2_RRM_sample_conditions
3D H(CCO)NH	SRSF2_RRM_sample_1	isotropic	SRSF2_RRM_sample_conditions
3D HCCH-TOCSY	SRSF2_RRM_sample_1	isotropic	SRSF2_RRM_sample_conditions
3D 1H-13C NOESY	SRSF2_RRM_sample_1	isotropic	SRSF2_RRM_sample_conditions
3D 1H-15N NOESY	SRSF2_RRM_sample_1	isotropic	SRSF2_RRM_sample_conditions
2D 1H-1H NOESY	SRSF2_RRM_D2O	isotropic	SRSF2_RRM_sample_conditions

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 600 MHz
Bruker Avance 700 MHz
Bruker Avance 900 MHz

BMRB	16444 17706 17707
PDB	2KN4 2LEA 2LEB 2LEC
DBJ	BAC03903 BAC04206 BAC36346 BAC39610 BAC40111
EMBL	CAA44306 CAA44307 CAA53383 CAA67134 CAJ82901
GB	AAA60306 AAC71000 AAH00339 AAH01303 AAH05493
PIR	A42701
PRF	1805195A 1805195B
REF	NP_001001305 NP_001009720 NP_001029290 NP_001029490 NP_001070697
SP	P30352 Q01130 Q06A98 Q3MHR5 Q5R1W5
TPG	DAA18198
AlphaFold	P30352 Q01130 Q06A98 Q3MHR5 Q5R1W5