BMRB Entry 17695

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17695

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Title:
Backbone Chemical Shift Assignments of the Extracellular domain of GLIC, a prokaryotic nAChR homologue
Deposition date:
2011-06-09
Original release date:
2012-01-04
Authors:
Chasapis, Christos; Argyriou, Aikaterini; Corringer, Pierre-Jean; Bentrop, Detlef; Spyroulias, Georgios
Citation:

Citation: Chasapis, Christos; Argyriou, Aikaterini; Corringer, Pierre-Jean; Bentrop, Detlef; Spyroulias, Georgios. "Unravelling the conformational plasticity of the extracellular domain of a prokaryotic nAChR homologue in solution by NMR"  Biochemistry 50, 9681-9683 (2011).
PubMed: 22007668

Assembly members:

Assembly members:
GLIC_ECD, polymer, 195 residues, 22079.9 Da.

Natural source:

Natural source:   Common Name: Gloeobacter violaceus   Taxonomy ID: 33072   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Gloeobacter violaceus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET20b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GLIC_ECD: MQDMVSPPPPIADEPLTVNT GIYLIECYSLDDKAETFKVN AFLSLSWKDRRLAFDPVRSG VRVKTYEPEAIWIPEIRFVN VENARDADVVDISVSPDGTV QYLERFSARVLSPLDGRRTE SDSQTLHIYLIVRSVDTRNI VLAVDLEKVGKNDDVFLTGW DIESFTAVVKPANFALEDRL ESKLDYQLRISRQGG

Data sets:
Data typeCount
13C chemical shifts354
15N chemical shifts144
1H chemical shifts144

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GLIC ECD1

Entities:

Entity 1, GLIC ECD 195 residues - 22079.9 Da.

The residues: 194 GLY and 195 GLY are cloning artifacts

1   METGLNASPMETVALSERPROPROPROPRO
2   ILEALAASPGLUPROLEUTHRVALASNTHR
3   GLYILETYRLEUILEGLUCYSTYRSERLEU
4   ASPASPLYSALAGLUTHRPHELYSVALASN
5   ALAPHELEUSERLEUSERTRPLYSASPARG
6   ARGLEUALAPHEASPPROVALARGSERGLY
7   VALARGVALLYSTHRTYRGLUPROGLUALA
8   ILETRPILEPROGLUILEARGPHEVALASN
9   VALGLUASNALAARGASPALAASPVALVAL
10   ASPILESERVALSERPROASPGLYTHRVAL
11   GLNTYRLEUGLUARGPHESERALAARGVAL
12   LEUSERPROLEUASPGLYARGARGTHRGLU
13   SERASPSERGLNTHRLEUHISILETYRLEU
14   ILEVALARGSERVALASPTHRARGASNILE
15   VALLEUALAVALASPLEUGLULYSVALGLY
16   LYSASNASPASPVALPHELEUTHRGLYTRP
17   ASPILEGLUSERPHETHRALAVALVALLYS
18   PROALAASNPHEALALEUGLUASPARGLEU
19   GLUSERLYSLEUASPTYRGLNLEUARGILE
20   SERARGGLNGLYGLY

Samples:

sample_1: GLIC ECD, [U-98% 13C; U-98% 15N], 0.6 mM; H2O 90%; D2O 10%; Pi 50 mM

sample_2: GLIC ECD, [U-100% 13C; U-100% 15N; U-80% 2H], 0.6 mM; H2O 90%; D2O 10%; Pi 50 mM

sample_3: GLIC ECD, [U-15N]-Leu, 0.6 mM; H2O 90%; D2O 10%; Pi 50 mM

sample_4-15: GLIC ECD, [U-15N], 0.3 – 0.5 mM; H2O 90%; D2O 10%; Pi 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 50 mM; pH: 7.2; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_2
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D C(CO)NHsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_4-15isotropicsample_conditions_1
2D 1H-15N HSQCsample_4-15isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 900 MHz

Related Database Links:

PDB
DBJ BAC92138
REF NP_927143 WP_011144181
SP Q7NDN8
AlphaFold Q7NDN8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks