BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17691

Title: Solution structure of the HMG box DNA-binding domain of human stem cell transcription factor Sox2

Deposition date: 2011-06-06 Original release date: 2011-07-06

Authors: Sahu, Sarata; Markley, John; Tonelli, Marco; Bahrami, Arash; Eghbalnia, Hamid

Citation: Sahu, Sarata; Markley, John; Tonelli, Marco; Bahrami, Arash; Eghbalnia, Hamid. "Not known"  .

Assembly members:
Sox2, polymer, 81 residues, 9920.689 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEU-HIS

Entity Sequences (FASTA):
Sox2: SDRVKRPMNAFMVWSRGQRR KMAQENPKMHNSEISKRLGA EWKLLSETEKRPFIDEAKRL RALHMKEHPDYKYRPRRKTK T

Data sets:
Data typeCount
13C chemical shifts370
15N chemical shifts80
1H chemical shifts532

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sox21

Entities:

Entity 1, Sox2 81 residues - 9920.689 Da.

1   SERASPARGVALLYSARGPROMETASNALA
2   PHEMETVALTRPSERARGGLYGLNARGARG
3   LYSMETALAGLNGLUASNPROLYSMETHIS
4   ASNSERGLUILESERLYSARGLEUGLYALA
5   GLUTRPLYSLEULEUSERGLUTHRGLULYS
6   ARGPROPHEILEASPGLUALALYSARGLEU
7   ARGALALEUHISMETLYSGLUHISPROASP
8   TYRLYSTYRARGPROARGARGLYSTHRLYS
9   THR

Samples:

sample_1: Sox2, [U-100% 13C; U-100% 15N], 0.7 ± 0.02 mM; DTT 5 ± 0.05 %; MOPS 10 mM; NaCl 100 mM; H20 95%; D20 5%

sample_conditions_1: ionic strength: 100 mM; pH: 6.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESY (AROMATIC)sample_1isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

PDB
DBJ BAA09168 BAC55017 BAC67545 BAC75668 BAE20882
EMBL CAA63847 CAA65725 CAA80631 CAA83435 CAF91617
GB AAA35997 AAB09662 AAB62821 AAC14215 AAC31791
PRF 1612347C
REF NP_001081691 NP_001098933 NP_001102651 NP_001116669 NP_001135190
SP O42569 P48430 P48431 P48432 P54231
TPG DAA33313
AlphaFold P48431