BMRB Entry 17685

Name: Solution structure of the C-terminal Pdr1 activating domain of Zuo1
Published: 2012-10-01

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PDB ID: 2lwx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17685
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the C-terminal Pdr1 activating domain of Zuo1

Deposition date:

2011-06-02

Original release date:

2012-10-01

Authors:

Volkman, B.; Waltner, J.; Peterson, F.

Citation:

Citation: Ducett, Jeanette; Peterson, Francis; Hoover, Lindsey; Prunuske, Amy; Volkman, Brian; Craig, Elizabeth. "Unfolding of the C-terminal domain of the J-protein Zuo1 releases autoinhibition and activates Pdr1-dependent transcription." J. Mol. Biol. 425, 19-31 (2013).
PubMed: 23036859

Assembly members:

Assembly members:
Zuo1, polymer, 108 residues, 11836.323 Da.

Natural source:

Natural source: Common Name: yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Zuo1: MRGSHHHHHHHHGSENLYFQ GSKAAKKKNKRAIRNSAKEA DYFGDADKATTIDEQVGLIV DSLNDEELVSTADKIKANAA GAKEVLKESAKTIVDSGKLP SSLLSYFV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	373
15N chemical shifts	93
1H chemical shifts	616

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Zuo1	1

Entities:

Entity 1, Zuo1 108 residues - 11836.323 Da.

The N-terminal residues 'MRGSHHHHHHHHGSENLYFQGS' are a cloning artifact.

1

MET

ARG

GLY

SER

HIS

2

HIS

GLY

SER

GLU

ASN

LEU

TYR

PHE

GLN

3

GLY

SER

LYS

ALA

LYS

ASN

LYS

4

ARG

ALA

ILE

ARG

ASN

SER

ALA

LYS

GLU

ALA

5

ASP

TYR

PHE

GLY

ASP

ALA

ASP

LYS

ALA

THR

6

THR

ILE

ASP

GLU

GLN

VAL

GLY

LEU

ILE

VAL

7

ASP

SER

LEU

ASN

ASP

GLU

LEU

VAL

SER

8

THR

ALA

ASP

LYS

ILE

LYS

ALA

ASN

ALA

9

GLY

ALA

LYS

GLU

VAL

LEU

LYS

GLU

SER

ALA

10

LYS

THR

ILE

VAL

ASP

SER

GLY

LYS

LEU

PRO

11

SER

LEU

SER

TYR

PHE

VAL

Samples:

sample: Zuo1, [U-100% 13C; U-100% 15N], 1.2 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D_13C-separated_NOESY (AROMATIC)	sample	isotropic	sample_conditions_1
3D_13C-separated_NOESY	sample	isotropic	sample_conditions_1
3D_15N-separated_NOESY	sample	isotropic	sample_conditions_1

Software:

X-PLOR NIH v2.9.3, SCHWIETERS,C.D.,KUSZEWSKI,J.J.,TJANDRA,N.,CLORE,G.M. - refinement

TOPSPIN v2.1, Bruker - collection

NMRPipe v2007, Delagio,F. et al. - processing

XEASY v1.3, Eccles, C., Guntert, P., Billeter, M., Wuthrich, K. - data analysis

GARANT v2.1, C. Bartels - data analysis

CYANA v2.1, Guntert, P. - structural calculation

NMR spectrometers:

Bruker Avance II 600 MHz

PDB	2LWX
DBJ	GAA23651
EMBL	CAY80044
GB	AHY79619 AJP39050 AJR76370 AJR76866 AJR77364