BMRB Entry 17668

Name: NMR solution structure of ZiaAN sub mutant
Published: 2011-11-22

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PDB ID: 2ldi
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17668
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR solution structure of ZiaAN sub mutant

Deposition date:

2011-05-26

Original release date:

2011-11-22

Authors:

Banci, Lucia; Bertini, Ivano; Felli, Isabella; Pavelkova, Anna

Citation:

Citation: Tottey, Steve; Patterson, Carl; Banci, Lucia; Bertini, Ivano; Felli, Isabella; Pavelkova, Anna; Dainty, Samantha; Pernil, Rafael; Waldron, Kevin; Foster, Andrew; Robinson, Nigel. "Cyanobacterial metallochaperone inhibits deleterious side reactions of copper." Proc. Natl. Acad. Sci. U.S.A. 109, 95-100 (2012).
PubMed: 22198771

Assembly members:

Assembly members:
ZiaAn sub mutant, polymer, 106 residues, 7586.802 Da.

Natural source:

Natural source: Common Name: Synechocystis sp. PCC 6803 Taxonomy ID: 1148 Superkingdom: Bacteria Kingdom: not available Genus/species: Synechocystis sp.

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ZiaAn sub mutant: PLKTQQMQVGGMRCAACASS IERALERLKGVAEASVTVAT GRLTVTYDPKQVSEITIQER IAALGYTLAEPKSSVTLNGH KHPHSHREEGHSHSHGAGEF NLKQEL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	403
15N chemical shifts	99
1H chemical shifts	512

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ZiaAn sub mutant	1

Entities:

Entity 1, ZiaAn sub mutant 106 residues - 7586.802 Da.

1

PRO

LEU

LYS

THR

GLN

MET

GLN

VAL

GLY

2

GLY

MET

ARG

CYS

ALA

CYS

ALA

SER

3

ILE

GLU

ARG

ALA

LEU

GLU

ARG

LEU

LYS

GLY

4

VAL

ALA

GLU

ALA

SER

VAL

THR

VAL

ALA

THR

5

GLY

ARG

LEU

THR

VAL

THR

TYR

ASP

PRO

LYS

6

GLN

VAL

SER

GLU

ILE

THR

ILE

GLN

GLU

ARG

7

ILE

ALA

LEU

GLY

TYR

THR

LEU

ALA

GLU

8

PRO

LYS

SER

VAL

THR

LEU

ASN

GLY

HIS

9

LYS

HIS

PRO

HIS

SER

HIS

ARG

GLU

GLY

10

HIS

SER

HIS

SER

HIS

GLY

ALA

GLY

GLU

PHE

11

ASN

LEU

LYS

GLN

GLU

LEU

Samples:

sample_1: ZiaAN sub, [U-99% 13C; U-99% 15N], 0.5 mM; sodium phosphate 50 mM; H20 90%; D20 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7.00; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v2, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 700 MHz
Bruker Avance 800 MHz