BMRB Entry 17645

Name: Solution structure of myosin VI lever arm extension
Published: 2012-08-30

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PDB ID: 2ld3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17645
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of myosin VI lever arm extension

Deposition date:

2011-05-13

Original release date:

2012-08-30

Authors:

Feng, Wei; Yu, Cong; Zhang, Mingjie

Citation:

Citation: Yu, Cong; Lou, Jizhong; Wu, Jingjing; Pan, Lifeng; Feng, Wei; Zhang, Mingjie. "Membrane-induced lever arm expansion allows myosin VI to walk with large and variable step sizes." J. Biol. Chem. 287, 35021-35035 (2012).
PubMed: 22936804

Assembly members:

Assembly members:
myosin_VI_LAE, polymer, 88 residues, 9961.641 Da.

Natural source:

Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
myosin_VI_LAE: QGPGSLVKVGTLKKRLDKFN EVVSALKDGKPEVNRQIKNL EISIDALMAKIKSTMMTREQ IQKEYDALVKSSEDLLSALQ KKKQQEEE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	305
15N chemical shifts	83
1H chemical shifts	637

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	myosin VI lever arm extension	1

Entities:

Entity 1, myosin VI lever arm extension 88 residues - 9961.641 Da.

Residues "QGPGS" are from the non-native tag

1

GLN

GLY

PRO

GLY

SER

LEU

VAL

LYS

VAL

GLY

2

THR

LEU

LYS

ARG

LEU

ASP

LYS

PHE

ASN

3

GLU

VAL

SER

ALA

LEU

LYS

ASP

GLY

LYS

4

PRO

GLU

VAL

ASN

ARG

GLN

ILE

LYS

ASN

LEU

5

GLU

ILE

SER

ILE

ASP

ALA

LEU

MET

ALA

LYS

6

ILE

LYS

SER

THR

MET

THR

ARG

GLU

GLN

7

ILE

GLN

LYS

GLU

TYR

ASP

ALA

LEU

VAL

LYS

8

SER

GLU

ASP

LEU

SER

ALA

LEU

GLN

9

LYS

GLN

GLU

Samples:

sample_1: myosin VI LAE 1.0 mM; D2O 100%; sodium phosphate 50 mM

sample_2: myosin VI LAE, [U-100% 15N], 1.0 mM; H2O 90%; D2O 10%

sample_3: myosin VI LAE, [U-100% 13C; U-100% 15N], 1.0 mM; H20 90%; D20 10%

sample_4: myosin VI LAE, [U-100% 13C; U-100% 15N], 1.0 mM; D2O 100%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_1
3D HNCO	sample_3	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_4	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

PIPP, Garrett - data analysis

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

Varian INOVA 500 MHz
Varian INOVA 750 MHz

PDB	2LD3
DBJ	BAD32219
GB	AAB00194 AAI44918 AAI50674 EDL26433 EDL26434
PRF	2204386A
REF	NP_001034635 XP_001061392 XP_003508949 XP_004696356 XP_004696357
SP	Q64331
AlphaFold	Q64331