BMRB Entry 17635

Name: solution structure of FUS/TLS RRM domain
Published: 2012-06-04

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PDB ID: 2lcw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17635
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

solution structure of FUS/TLS RRM domain

Deposition date:

2011-05-10

Original release date:

2012-06-04

Authors:

Liu, Xuehui; Ren, Jintao; Niu, Chunyan; Gong, Weimin; Feng, Wei

Citation:

Citation: Liu, Xuehui; Ren, Jintao; Niu, Chunyan; Feng, Wei; Gong, Weimin. "TLS-RRM is a promiscuous nucleic acid binding domain" .

Assembly members:

Assembly members:
FUS/TLS RRM domain, polymer, 108 residues, 11747.013 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FUS/TLS RRM domain: EQDNSDNNTIFVQGLGENVT IESVADYFKQIGIIKTNKKT GQPMINLYTDRETGKLKGEA TVSFDDPPSAKAAIDWFDGK EFSGNPIKVSFATRRADFNR GGGNGRGG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	446
15N chemical shifts	117
1H chemical shifts	657

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FUS/TLS RRM domain	1

Entities:

Entity 1, FUS/TLS RRM domain 108 residues - 11747.013 Da.

1

GLU

GLN

ASP

ASN

SER

ASP

ASN

THR

ILE

2

PHE

VAL

GLN

GLY

LEU

GLY

GLU

ASN

VAL

THR

3

ILE

GLU

SER

VAL

ALA

ASP

TYR

PHE

LYS

GLN

4

ILE

GLY

ILE

LYS

THR

ASN

LYS

THR

5

GLY

GLN

PRO

MET

ILE

ASN

LEU

TYR

THR

ASP

6

ARG

GLU

THR

GLY

LYS

LEU

LYS

GLY

GLU

ALA

7

THR

VAL

SER

PHE

ASP

PRO

SER

ALA

8

LYS

ALA

ILE

ASP

TRP

PHE

ASP

GLY

LYS

9

GLU

PHE

SER

GLY

ASN

PRO

ILE

LYS

VAL

SER

10

PHE

ALA

THR

ARG

ALA

ASP

PHE

ASN

ARG

11

GLY

ASN

GLY

ARG

GLY

Samples:

sample_1: DSS 0.5 mM; Tris 20 mM; NaCl 50 mM; H2O 10%; D2O, [U-100% 2H], 90%; NaN3 0.02%; FUS_TLS_RRM_domain, [U-100% 15N; U-100% 13C], 1 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

Bruker DRX 600 MHz

PDB	2LCW
DBJ	BAD92139 BAE24196 BAE26524 BAE26972 BAE87186
EMBL	CAA50559 CAG33028 CAJ83479
GB	AAA79948 AAB27102 AAC13543 AAC35284 AAC35285
PRF	1916411B
REF	NP_001001531 NP_001012137 NP_001080383 NP_001086293 NP_001090836
SP	P35637 P56959 Q28009
TPG	DAA15352
AlphaFold	P35637 P56959 Q28009