BMRB Entry 17631

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for human JAZ ZF2
Published: 2014-02-24

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17631

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for human JAZ ZF2

Deposition date:

2011-05-08

Original release date:

2014-02-24

Authors:

Burge, Russell; Martinez-Yamout, Maria; Gottesfeld, Joel; Dyson, H.; Wright, Peter; Dyson, Jane

Citation:

Citation: Burge, Russell; Martinez-Yamout, Maria; Gottesfeld, Joel; Dyson, H.; Wright, Peter. "Backbone assignment of human JAZ ZF2" Biochemistry ., .-..

Assembly members:

Assembly members:
JAZ_ZF2, polymer, 55 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
JAZ_ZF2: GETKKLDSDQKSSRSKDKNQ CCPICNMTFSSPVVAQSHYL GKTHAKNLKLKQQST

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	80
15N chemical shifts	45
1H chemical shifts	45

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	JAZ ZF2	1
2	zinc ion	2

Entities:

Entity 1, JAZ ZF2 55 residues - Formula weight is not available

1

GLY

GLU

THR

LYS

LEU

ASP

SER

ASP

GLN

2

LYS

SER

ARG

SER

LYS

ASP

LYS

ASN

GLN

3

CYS

PRO

ILE

CYS

ASN

MET

THR

PHE

SER

4

SER

PRO

VAL

ALA

GLN

SER

HIS

TYR

LEU

5

GLY

LYS

THR

HIS

ALA

LYS

ASN

LEU

LYS

LEU

6

LYS

GLN

SER

THR

Entity 2, zinc ion - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: JAZ ZF2, [U-98% 13C; U-98% 15N], 300 uM; TRIS 25 mM; potassium chloride 50 mM; D2O, [U-99% 2H], 7%; DTT 2 mM; zinc 50 uM; H2O 90%

sample_conditions_1: ionic strength: 75 mM; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRView v5, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker DRX 600 MHz

GB	EAW85038 AAD52018 AAH07775 AIC50988 EAW85038 EAW85040
DBJ	BAD18614 BAG72878
EMBL	CAH18475 CAL37677 CAL38643
REF	NP_001231409 NP_001295142 NP_001295144 NP_036411 XP_001498579
SP	Q9UL40
AlphaFold	Q9UL40