BMRB Entry 17630

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Human Just Another Zinc Finger Protein residues 23-117
Published: 2014-02-24

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17630

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Human Just Another Zinc Finger Protein residues 23-117

Deposition date:

2011-05-08

Original release date:

2014-02-24

Authors:

Burge, Russell; Martinez-Yamout, Maria; Gottesfeld, Joel; Dyson, H.; Wright, Peter; Dyson, Jane

Citation:

Citation: Burge, Russell; Martinez-Yamout, Maria; Dyson, H. Jane; Wright, Peter. "Structural Characterization of Interactions between the Double-Stranded RNA-Binding Zinc Finger Protein JAZ and Nucleic Acids." Biochemistry ., .-. (2014).
PubMed: 24521053

Assembly members:

Assembly members:
JAZ_ZF1, polymer, 95 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
JAZ_ZF1: SELLEGQEPDGVRFDRERAR RLWEAVSGAQPVGREEVEHM IQKNQCLFTNTQCKVCCALL ISESQKLAHYQSKKHANKVK RYLAIHGMETLKGET

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	72
15N chemical shifts	64
1H chemical shifts	65

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	JAZ ZF1	1
2	zinc ion	2

Entities:

Entity 1, JAZ ZF1 95 residues - Formula weight is not available

1

SER

GLU

LEU

GLU

GLY

GLN

GLU

PRO

ASP

2

GLY

VAL

ARG

PHE

ASP

ARG

GLU

ARG

ALA

ARG

3

ARG

LEU

TRP

GLU

ALA

VAL

SER

GLY

ALA

GLN

4

PRO

VAL

GLY

ARG

GLU

VAL

GLU

HIS

MET

5

ILE

GLN

LYS

ASN

GLN

CYS

LEU

PHE

THR

ASN

6

THR

GLN

CYS

LYS

VAL

CYS

ALA

LEU

7

ILE

SER

GLU

SER

GLN

LYS

LEU

ALA

HIS

TYR

8

GLN

SER

LYS

HIS

ALA

ASN

LYS

VAL

LYS

9

ARG

TYR

LEU

ALA

ILE

HIS

GLY

MET

GLU

THR

10

LEU

LYS

GLY

GLU

THR

Entity 2, zinc ion - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: JAZ ZF1, [U-99% 13C; U-99% 15N], 250 – 300 uM; TRIS 25 mM; potassium chloride 50 mM; D2O, [U-99% 2H], 7%; DTT 2 mM; zinc 50 uM; H2O 93%

sample_conditions_1: ionic strength: 75 mM; pH: 7.3; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific - chemical shift assignment, processing

NMR spectrometers:

Bruker DRX 600 MHz

GB	EAW85038 AAD52018 AAH07775 AIC50988 EAW85038 EAW85039
DBJ	BAG72878 BAH13202 BAH13338
EMBL	CAH18475 CAH93198 CAL37677 CAL38643
REF	NP_001126881 NP_001295144 NP_001295145 NP_001295148 NP_036411
SP	Q5R4W8 Q9UL40
AlphaFold	Q5R4W8 Q9UL40