BMRB Entry 17620

Name: 1H,15N and 13C Assignment of the D2 domain of the Human Fibroblast Growth Factor Receptor 4
Published: 2011-06-03

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17620

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H,15N and 13C Assignment of the D2 domain of the Human Fibroblast Growth Factor Receptor 4

Deposition date:

2011-05-03

Original release date:

2011-06-03

Authors:

Elshorst, Bettina; Saxena, Krishna; Schieborr, Ulrich; Schwalbe, Harald

Citation:

Citation: Elshorst, Bettina; Saxena, Krishna; Schieborr, Ulrich; Schwalbe, Harald. "1H, 13C and 15N assignment of D2 domain of human fibroblast growth factor receptor 4." Biomol. NMR Assignments 7, 179-182 (2013).
PubMed: 22752790

Assembly members:

Assembly members:
FGFR4_D2, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FGFR4_D2: MNRHSYPQQAPYWTHPQRME KKLHAVPAGNTVKFRCPAAG NPTPTIRWLKDGQAFHGENR IGGIRLRHQHWSLVMESVVP SDRGTYTCLVENAVGSIRYN YLLDVLLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	343
15N chemical shifts	87
1H chemical shifts	546

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FGFR4 D2	1

Entities:

Entity 1, FGFR4 D2 114 residues - Formula weight is not available

1

MET

ASN

ARG

HIS

SER

TYR

PRO

GLN

ALA

2

PRO

TYR

TRP

THR

HIS

PRO

GLN

ARG

MET

GLU

3

LYS

LEU

HIS

ALA

VAL

PRO

ALA

GLY

ASN

4

THR

VAL

LYS

PHE

ARG

CYS

PRO

ALA

GLY

5

ASN

PRO

THR

PRO

THR

ILE

ARG

TRP

LEU

LYS

6

ASP

GLY

GLN

ALA

PHE

HIS

GLY

GLU

ASN

ARG

7

ILE

GLY

ILE

ARG

LEU

ARG

HIS

GLN

HIS

8

TRP

SER

LEU

VAL

MET

GLU

SER

VAL

PRO

9

SER

ASP

ARG

GLY

THR

TYR

THR

CYS

LEU

VAL

10

GLU

ASN

ALA

VAL

GLY

SER

ILE

ARG

TYR

ASN

11

TYR

LEU

ASP

VAL

LEU

GLU

HIS

12

HIS

Samples:

sample_1: FGFR4 D2, [U-99% 13C; U-99% 15N], 0.7 mM; BIS-TRIS 25 mM; sodium chloride 200 mM; arginine 25 mM; TSP 1 mM; H2O 90%; D2O 10%

sample_2: FGFR4 D2, [U-99% 13C; U-99% 15N], 0.7 mM; BIS-TRIS 25 mM; sodium chloride 200 mM; arginine 25 mM; TSP 1 mM; D2O 100%

sample_conditions_1: ionic strength: 200 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HMQC	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D CCH-TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_1

Software:

CARA, Rochus Keller - chemical shift assignment

NMR spectrometers:

Bruker DRX 800 MHz
Bruker DRX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks