BMRB Entry 17601

Title:
Solution structure of the MLV readthrough pseudoknot   PubMed: 22121021
Deposition date:
2011-04-26
Original release date:
2011-12-02
Authors:
Houck-Loomis, Brian; Durney, Michael; D'Souza, Victoria
Citation:

Citation: Houck-Loomis, Brian; Durney, Michael; Salguero, Carolina; Shankar, Neelaabh; Nagle, Julia; Goff, Stephen. "An equilibrium-dependent retroviral mRNA switch regulates translational recoding."  Nature 480, 561-564 (2011).

Assembly members:

Assembly members:
RNA_(59-MER), polymer, 63 residues, 111.103 Da.

Natural source:

Natural source:   Common Name: MMLV   Taxonomy ID: 11801   Superkingdom: Viruses   Kingdom: not available   Genus/species: not available murine leukemia virus

Experimental source:   Production method: recombinant technology   Host organism: None: in vitro transcription   Vector: pUC19

Experimental source:

Natural source:   Common Name: MMLV   Taxonomy ID: 11801   Superkingdom: Viruses   Kingdom: not available   Genus/species: not available murine leukemia virus

Experimental source:   Production method: recombinant technology   Host organism: None: in vitro transcription   Vector: pUC19

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts92
1H chemical shifts182

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA_(59-MER)1

Entities:

Entity 1, RNA_(59-MER) 63 residues - 111.103 Da.

1   GGAGGUCAGG
2   GUCAGGAGCC
3   CCCCCCUGAA
4   CCCAGGAUAA
5   CCCUCAAAGU
6   CGGGGGGCAA
7   CCC

Samples:

MLV-PK: RNA (59-MER)0.2 – 2.0 mM; D2O 100%

MLV-PK-AC-13C_15N: RNA (59-MER), [U-100% 13C; U-100% 15N], 0.2 – 2.0 mM; D2O 100%

MLV-PK-AC-2H: RNA (59-MER), [U-100% 2H], 0.2 – 2.0 mM; D2O 100%

sample_conditions_1: ionic strength: 0.02 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYMLV-PKisotropicsample_conditions_1
2D 1H-13C HSQCMLV-PK-AC-13C_15Nisotropicsample_conditions_1
2D 1H-13C HSQCMLV-PK-AC-13C_15Nisotropicsample_conditions_1
2D 1H-1H NOESYMLV-PK-AC-2Hisotropicsample_conditions_1
2D 1H-1H NOESYMLV-PK-AC-13C_15Nisotropicsample_conditions_1
2D 1H-1H NOESYMLV-PKisotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 900 MHz