BMRB Entry 17598

Name: Solution Structure of PilP from Pseudomonas aeruginosa
Published: 2011-12-20

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PDB ID: 2lc4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17598
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of PilP from Pseudomonas aeruginosa

Deposition date:

2011-04-22

Original release date:

2011-12-20

Authors:

Howell, P. Lynne; Tammam, Stephanie; Chong, P. Andrew; Forman-Kay, Julie

Citation:

Citation: Tammam, Stephanie; Sampaleanu, Liliana; Koo, Jason; Sundaram, Priyanka; Ayers, Melissa; Chong, P. Andrew; Forman-Kay, Julie; Burrows, Lori; Howell, P. Lynne. "Characterization of the PilN, PilO and PilP type IVa pilus subcomplex" Mol. Micro. 82, 1496-1514 (2011).
PubMed: 22053789

Assembly members:

Assembly members:
PilP, polymer, 111 residues, 12427.167 Da.

Natural source:

Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PilP: DLTVRQKGNKVIKPDETRVK QFLEGFNIETFEMVGTLSNA QGTFALVKGAGGVHRVRVGD YLGRNDGKVVGISEGKIDVI EIVPDGEGNWLERPRSLTLK ERSLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	367
15N chemical shifts	107
1H chemical shifts	741

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PilP	1

Entities:

Entity 1, PilP 111 residues - 12427.167 Da.

1

ASP

LEU

THR

VAL

ARG

GLN

LYS

GLY

ASN

LYS

2

VAL

ILE

LYS

PRO

ASP

GLU

THR

ARG

VAL

LYS

3

GLN

PHE

LEU

GLU

GLY

PHE

ASN

ILE

GLU

THR

4

PHE

GLU

MET

VAL

GLY

THR

LEU

SER

ASN

ALA

5

GLN

GLY

THR

PHE

ALA

LEU

VAL

LYS

GLY

ALA

6

GLY

VAL

HIS

ARG

VAL

ARG

VAL

GLY

ASP

7

TYR

LEU

GLY

ARG

ASN

ASP

GLY

LYS

VAL

8

GLY

ILE

SER

GLU

GLY

LYS

ILE

ASP

VAL

ILE

9

GLU

ILE

VAL

PRO

ASP

GLY

GLU

GLY

ASN

TRP

10

LEU

GLU

ARG

PRO

ARG

SER

LEU

THR

LEU

LYS

11

GLU

ARG

SER

LEU

GLU

HIS

12

HIS

Samples:

sample_1: sodium chloride 150 mM; TRIS 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCC-TOCSY	sample_1	isotropic	sample_conditions_1
3D CCC-TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

TALOS, Cornilescu, Delaglio and Bax - geometry optimization

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

Varian INOVA 500 MHz
Varian INOVA 600 MHz

PDB	2LC4
DBJ	BAK87361 BAP24857 BAP53627 BAQ42890 BAR70506
EMBL	CAW30185 CCQ86063 CDH73786 CDH80106 CDI93614
GB	AAA87405 AAG08426 ABJ14425 ABR86649 AEO77634
REF	NP_253728 WP_003095836 WP_003116136 WP_012077721 WP_021263517