BMRB Entry 17596

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17596
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone and sidechain 1H, 13C and 15N chemical shift assignments of the hydrophobin DewA from Aspergillus nidulans
Deposition date:
2011-04-20
Original release date:
2011-08-19
Authors:
Morris, Vanessa; Kwan, Ann; Mackay, Joel; Sunde, Margaret
Citation:

Citation: Morris, Vanessa; Kwan, Ann; Mackay, Joel; Sunde, Margaret. "Backbone and sidechain (1)H, (13)C and (15)N chemical shift assignments of the hydrophobin DewA from Aspergillus nidulans."  Biomol. NMR Assignments 6, 83-86 (2012).
PubMed: 21845363

Assembly members:

Assembly members:
DewA, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Aspergillus nidulans   Taxonomy ID: 162425   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Aspergillus nidulans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHUE

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DewA: SLPASAAKNAKLATSAAFAK QAEGTTCNVGSIACCNSPAE TNNDSLLSGLLGAGLLNGLS GNTGSACAKASLIDQLGLLA LVDHTEEGPVCKNIVACCPE GTTNCVAVDNAGAGTKAE

Data sets:
Data typeCount
13C chemical shifts854
15N chemical shifts238
1H chemical shifts1382

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DewA1

Entities:

Entity 1, DewA 118 residues - Formula weight is not available

Residue 1 is a cloning artefact. Our construct starts from residue 19 of the native precursor, which is at the cleavage site.

1   SERLEUPROALASERALAALALYSASNALA
2   LYSLEUALATHRSERALAALAPHEALALYS
3   GLNALAGLUGLYTHRTHRCYSASNVALGLY
4   SERILEALACYSCYSASNSERPROALAGLU
5   THRASNASNASPSERLEULEUSERGLYLEU
6   LEUGLYALAGLYLEULEUASNGLYLEUSER
7   GLYASNTHRGLYSERALACYSALALYSALA
8   SERLEUILEASPGLNLEUGLYLEULEUALA
9   LEUVALASPHISTHRGLUGLUGLYPROVAL
10   CYSLYSASNILEVALALACYSCYSPROGLU
11   GLYTHRTHRASNCYSVALALAVALASPASN
12   ALAGLYALAGLYTHRLYSALAGLU

Samples:

15N_DewA: DewA, [U-99% 15N], 300 – 500 uM; DSS 0.1 mM; sodium acetate 20 mM; H20 95%; D20 5%

15N13C_DewA: DewA, [U-98% 13C; U-98% 15N], 300 – 500 uM; DSS 0.1 mM; sodium acetate 20 mM; H20 95%; D20 5%

15N13C_DewA_D2O: DewA, [U-98% 13C; U-98% 15N], 300 – 500 uM; DSS 0.1 mM; sodium acetate 20 mM; H20 95%; D20 5%

unlabelled_DewA: DewA, [U-98% 13C; U-98% 15N], 300 – 500 uM; DSS 0.1 mM; sodium acetate 20 mM; H20 95%; D20 5%

sample_conditions_1: ionic strength: 40 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 40 mM; pH: 5.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC15N13C_DewAisotropicsample_conditions_1
2D 1H-13C HSQC15N13C_DewAisotropicsample_conditions_1
2D 1H-1H NOESYunlabelled_DewAisotropicsample_conditions_1
3D CBCA(CO)NH15N13C_DewAisotropicsample_conditions_1
3D C(CO)NH15N13C_DewAisotropicsample_conditions_1
3D HNCO15N13C_DewAisotropicsample_conditions_1
3D HNCACB15N13C_DewAisotropicsample_conditions_1
3D HBHA(CO)NH15N13C_DewAisotropicsample_conditions_1
3D HN(CA)CO15N13C_DewAisotropicsample_conditions_1
3D 1H-15N NOESY15N_DewAisotropicsample_conditions_1
3D 1H-15N TOCSY15N_DewAisotropicsample_conditions_1
3D 1H-13C NOESY aliphatic15N13C_DewA_D2Oisotropicsample_conditions_2
3D HCCH-TOCSY15N13C_DewA_D2Oisotropicsample_conditions_2

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB AAC13762 EAA58809
REF XP_681275
SP P52750
TPE CBF73692
AlphaFold P52750

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks