BMRB Entry 17592

Name: Backbone assignments of Anabaena Sensory Rhodopsin Transducer with DNA
Published: 2011-06-22

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17592

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignments of Anabaena Sensory Rhodopsin Transducer with DNA

Deposition date:

2011-04-13

Original release date:

2011-06-22

Authors:

Wang, Shenlin; Kim, So Young; Jung, Kwang-Hwan; Ladizhansky, Vladimir; Brown, Leonid

Citation:

Citation: Wang, Shenlin; Kim, So Young; Jung, Kwang-Hwan; Ladizhansky, Vladimir; Brown, Leonid. "A eukaryotic-like interaction of soluble cyanobacterial sensory rhodopsin transducer with DNA." J. Mol. Biol. 411, 449-462 (2011).
PubMed: 21683709

Assembly members:

Assembly members:
ASRT, polymer, 124 residues, Formula weight is not available
DNA, polymer, 20 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Anabaena variabilis Taxonomy ID: 1172 Superkingdom: Bacteria Kingdom: not available Genus/species: Anabaena variabilis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Codon Plus

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ASRT: SLSIGRTCWAIAEGYIPPYG NGPEPQFISHETVCILNAGD EDAHVEITIYYSDKEPVGPY RLTVPARRTKHVRFNDLNDP APIPHDTDFASVIQSNVPIV VQHTRLDSRQAENALLSTIA YANT
DNA: ATGACCTTTAGGAGGAAAGA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	306
15N chemical shifts	93
1H chemical shifts	93

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Anabaena Sensory Rhodopsin Transducer	1
2	DNA	2

Entities:

Entity 1, Anabaena Sensory Rhodopsin Transducer 124 residues - Formula weight is not available

1

SER

LEU

SER

ILE

GLY

ARG

THR

CYS

TRP

ALA

2

ILE

ALA

GLU

GLY

TYR

ILE

PRO

TYR

GLY

3

ASN

GLY

PRO

GLU

PRO

GLN

PHE

ILE

SER

HIS

4

GLU

THR

VAL

CYS

ILE

LEU

ASN

ALA

GLY

ASP

5

GLU

ASP

ALA

HIS

VAL

GLU

ILE

THR

ILE

TYR

6

TYR

SER

ASP

LYS

GLU

PRO

VAL

GLY

PRO

TYR

7

ARG

LEU

THR

VAL

PRO

ALA

ARG

THR

LYS

8

HIS

VAL

ARG

PHE

ASN

ASP

LEU

ASN

ASP

PRO

9

ALA

PRO

ILE

PRO

HIS

ASP

THR

ASP

PHE

ALA

10

SER

VAL

ILE

GLN

SER

ASN

VAL

PRO

ILE

VAL

11

VAL

GLN

HIS

THR

ARG

LEU

ASP

SER

ARG

GLN

12

ALA

GLU

ASN

ALA

LEU

SER

THR

ILE

ALA

13

TYR

ALA

ASN

THR

Entity 2, DNA 20 residues - Formula weight is not available

1		DA	DT	DG	DA	DC	DC	DT	DT	DT	DA
2		DG	DG	DA	DG	DG	DA	DA	DA	DG	DA

Samples:

sample_1: ASRT, [U-100% 13C; U-100% 15N; U-50% 2H], 1 mM; H20 90%; D20 10%; Tris 50 mM; NaCl 50 mM

sample_2: ASRT, [U-100% 15N], 1 mM; H20 90%; D20 10%; Tris 50 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.2; pressure: 1 atm; temperature: 308 K

sample_conditions_2: ionic strength: 50 mM; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_2
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment, data analysis

NMR spectrometers:

Bruker Avance 600 MHz

BMRB	17590 17591
PDB	2II7 2II8 2II9 2IIA
DBJ	BAB74865
GB	ABA23469
REF	WP_010997317