BMRB Entry 17573

Title:
Conformation Effects of Base Modification on the Anticodon Stem-loop of Bacillus subtilis tRNATYR   PubMed: 21782828
Deposition date:
2011-04-05
Original release date:
2011-08-02
Authors:
Denmon, Andria; Wang, Jiachen; Nikonowicz, Edward
Citation:

Citation: Denmon, Andria; Wang, Jiachen; Nikonowicz, Edward. "Conformation effects of base modification on the anticodon stem-loop of Bacillus subtilis tRNA(Tyr)."  J. Mol. Biol. 412, 285-303 (2011).

Assembly members:

Assembly members:
coi6y, polymer, 17 residues, 417.358 Da.

Natural source:

Natural source:   Common Name: B. subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: enzymatic semisynthesis   Host organism: cell free   Vector: n/a

Experimental source:

Natural source:   Common Name: B. subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: enzymatic semisynthesis   Host organism: cell free   Vector: n/a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
coi6y: GGGGACUGUAXAXCCCC

Data sets:
Data typeCount
13C chemical shifts38
1H chemical shifts75

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1coi6y1

Entities:

Entity 1, coi6y 17 residues - 417.358 Da.

1   GGGGACUGUA
2   6IAAPSUCCCC

Samples:

sample_1: i6A,PSU-ASL 2.0 mM; potassium chloride 10 mM; EDTA 0.02 mM; potassium phosphate 10 mM; D2O 100%

sample_conditions_1: pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
Natural abundance 2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

FELIX, Accelrys Software Inc. - data analysis, processing

InsightII, Accelrys Software Inc. - data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz