BMRB Entry 17572

Title:
i6A37_tyrASL   PubMed: 21782828
Deposition date:
2011-04-05
Original release date:
2011-08-02
Authors:
Denmon, Andria; Wang, Jiachen; Nikonowicz, Edward
Citation:

Citation: Denmon, Andria; Wang, Jiachen; Nikonowicz, Edward. "Conformation effects of base modification on the anticodon stem-loop of Bacillus subtilis tRNA(Tyr)."  J. Mol. Biol. 412, 285-303 (2011).

Assembly members:

Assembly members:
i6A37_tyrASL, polymer, 17 residues, 417.358 Da.

Natural source:

Natural source:   Common Name: B. subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: not applicable

Experimental source:

Natural source:   Common Name: B. subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: not applicable

Entity Sequences (FASTA):

Entity Sequences (FASTA):
i6A37_tyrASL: GGGGACUGUAXAUCCCC

Data sets:
Data typeCount
13C chemical shifts101
15N chemical shifts24
1H chemical shifts143

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1i6A37 tyrASL1

Entities:

Entity 1, i6A37 tyrASL 17 residues - 417.358 Da.

1   GGGGACUGUA
2   6IAAUCCCC

Samples:

sample_1: i6A tyrASL, [U-100% 13C; U-100% 15N], 2.0 mM; potassium phosphate 10 mM; potassium chloride 10 mM; EDTA 0.02 mM; D2O 100%

sample_conditions_1: pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D HCCH-COSYsample_1isotropicsample_conditions_1
2D 1H-13C Long Range HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D HCCH-RELAYsample_1isotropicsample_conditions_1
2D HCNCsample_1isotropicsample_conditions_1

Software:

FELIX, Accelrys Software Inc. - data analysis, processing

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

InsightII, Accelrys Software Inc. - data analysis

VNMRJ, Varian - collection

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz