BMRB Entry 17571

Name: (Revised) SOLUTION STRUCTURE OF THE MONOMERIC FORM OF A MUTANT UNLIGANDED BOVINE NEUROPHYSIN, 20 STRUCTURES.
Published: 2012-04-03

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PDB ID: 2lbn
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17571
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

(Revised) SOLUTION STRUCTURE OF THE MONOMERIC FORM OF A MUTANT UNLIGANDED BOVINE NEUROPHYSIN, 20 STRUCTURES.

Deposition date:

2011-04-01

Original release date:

2012-04-03

Authors:

Lee, Hunjoong; Nguyen, Tam; Bracken, Clay; Breslow, Esther

Citation:

Citation: Lee, Hunjoong; Naik, Mandar; Bracken, Clay; Breslow, Esther. "Structural Basis of the Dimerization-Induced Increase in Neurophysin-Hormone Affinity: Interplay of Inter-Domain and Inter-Subunit Interactions" .

Assembly members:

Assembly members:
Mutant Unliganded Bovine Neurophysin, polymer, 92 residues, 9286.461 Da.

Natural source:

Natural source: Common Name: cow Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: T7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Mutant Unliganded Bovine Neurophysin: AVLDLDVRTCLPCGPGGKGR CFGPSICCGDELGCFVGTAE ALRCQEENYLPSPCQSGQKP CGSGGRCAAAGICCSPDGCE EDPACDPEAAFS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	170
15N chemical shifts	74
1H chemical shifts	367

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Mutant Unliganded Bovine Neurophysin	1

Entities:

Entity 1, Mutant Unliganded Bovine Neurophysin 92 residues - 9286.461 Da.

1

ALA

VAL

LEU

ASP

LEU

ASP

VAL

ARG

THR

CYS

2

LEU

PRO

CYS

GLY

PRO

GLY

LYS

GLY

ARG

3

CYS

PHE

GLY

PRO

SER

ILE

CYS

GLY

ASP

4

GLU

LEU

GLY

CYS

PHE

VAL

GLY

THR

ALA

GLU

5

ALA

LEU

ARG

CYS

GLN

GLU

ASN

TYR

LEU

6

PRO

SER

PRO

CYS

GLN

SER

GLY

GLN

LYS

PRO

7

CYS

GLY

SER

GLY

ARG

CYS

ALA

8

GLY

ILE

CYS

SER

PRO

ASP

GLY

CYS

GLU

9

GLU

ASP

PRO

ALA

CYS

ASP

PRO

GLU

ALA

10

PHE

SER

Samples:

sample_1: entity, [U-100% 15N], 1.4 mM; H2O 90%; D2O 10%

sample_2: entity, [U-100% 13C; U-100% 15N], 1.4 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 283 K

sample_conditions_2: pH: 7.5; pressure: 1 atm; temperature: 298 K

sample_conditions_3: pH: 7.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_2
3D HNHA	sample_2	isotropic	sample_conditions_3
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_3
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_3
2D 1H-15N HSQC	sample_2	anisotropic	sample_conditions_3

Software:

CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMRView, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	17561
PDB	1L5C 1L5D 2HNU 2HNV 2HNW 2LBH 2LBN
DBJ	BAK09299 BAK09301 BAK09303
EMBL	CAA23448 CAA23450 CAA25462 CAJ81049
GB	AAA30680 AAI41998 AFU54450 ELK04017
PRF	0904308A 1005247A 1307200B
REF	NP_789825 XP_006042791
SP	P01175
TPG	DAA23107
AlphaFold	P01175