BMRB Entry 17563

Title:
Glycyl-tRNA(GCC) anticodon stem-loop from Bacillus subtilis
Deposition date:
2011-03-31
Original release date:
2012-04-04
Authors:
Chang, Andrew; Nikonowicz, Edward
Citation:

Citation: Chang, Andrew; Nikonowicz, Edward. "Structure solution of three glycyl-tRNA anticodon stem-loops"  .

Assembly members:

Assembly members:
glycyl-tRNA, polymer, 17 residues, 5434.313 Da.

Natural source:

Natural source:   Common Name: B. subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: enzymatic semisynthesis   Host organism: Bacillus subtilis

Experimental source:

Natural source:   Common Name: B. subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: enzymatic semisynthesis   Host organism: Bacillus subtilis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
glycyl-tRNA: GGGCCUUGCCAAGGUCC

Data sets:
Data typeCount
13C chemical shifts90
1H chemical shifts108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA (5'-R(*GP*GP*GP*CP*CP*UP*UP*GP*CP*CP*AP*AP*GP*GP*UP*CP*C)-3')1

Entities:

Entity 1, RNA (5'-R(*GP*GP*GP*CP*CP*UP*UP*GP*CP*CP*AP*AP*GP*GP*UP*CP*C)-3') 17 residues - 5434.313 Da.

1   GGGCCUUGCC
2   AAGGUCC

Samples:

sample_1: RNA, [U-98% 13C; U-98% 15N], 0.02 – 0.04 mM; D2O 100%; KH2PO4 10 mM; K2HPO4 10 mM; KCl 10 mM; EDTA 0.2 mM

sample_2: RNA0.02 – 0.04 mM; D2O 100%; KH2PO4 10 mM; K2HPO4 10 mM; KCl 10 mM; EDTA 0.2 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D HCNsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

FELIX, Accelrys Software Inc. - chemical shift assignment, processing

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz