BMRB Entry 17559

Name: Assignment of the stem loop 2 of RsmZ
Published: 2012-01-24

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17559

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignment of the stem loop 2 of RsmZ

Deposition date:

2011-03-31

Original release date:

2012-01-24

Authors:

Schubert, Mario; Aeschbacher, Thomas; Duss, Olivier; Allain, Frederic

Citation:

Citation: Aeschbacher, Thomas; Schubert, Mario; Allain, Frederic H-T. "A procedure to validate and correct the (13)C chemical shift calibration of RNA datasets." J. Biomol. NMR 52, 179-190 (2012).
PubMed: 22252483

Assembly members:

Assembly members:
FZL2, polymer, 22 residues, Formula weight is not available

Natural source:

Natural source: Common Name: g-proteobacteria Taxonomy ID: 294 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas fluorescens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: not applicable Vector: not applicable

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FZL2: GGGCCAUCAAGGACGAUGGU CC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	59
1H chemical shifts	59

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FZL2 (monomer)	1

Entities:

Entity 1, FZL2 (monomer) 22 residues - Formula weight is not available

1

G

C

A

U

C

A

2

G

A

C

G

A

U

G

U

3

C

Samples:

sample_1: FZL2 2 mM; D2O 100%

sample_2: FZL2 2 mM; D2O 5%; H2O 95%

sample_conditions_1: ionic strength: 0 M; pH: 7.4; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, refinement

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 600 MHz
Bruker Avance 900 MHz