BMRB Entry 17539

Name: Structure of first WW domain of human YAP in complex with a human Smad1 doubly-phosphorylated derived peptide.
Published: 2011-06-23

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PDB ID: 2lax
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17539
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of first WW domain of human YAP in complex with a human Smad1 doubly-phosphorylated derived peptide.

Deposition date:

2011-03-22

Original release date:

2011-06-23

Authors:

Macias, Maria; Aragon, Eric; Goerner, Nina; Zaromytidou, Alexia-Ileana; Xi, Qiaoran; Escobedo, Albert; Massague, Joan

Citation:

Citation: Aragon, Eric; Goerner, Nina; Zaromytidou, Alexia-Ileana; Xi, Qiaoran; Escobedo, Albert; Massague, Joan; Macias, Maria. "A Smad action turnover switch operated by WW domain readers of a phosphoserine code." Genes Dev. 25, 1275-1288 (2011).
PubMed: 21685363

Assembly members:

Assembly members:
first WW domain of human YAP, polymer, 40 residues, 4151.635 Da.
Smad1 doubly-phosphorilated derived peptide, polymer, 9 residues, 1136.976 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: petM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
first WW domain of human YAP: GAMEDVPLPAGWEMAKTSSG QRYFLNHIDQTTTWQDPRKA
Smad1 doubly-phosphorilated derived peptide: SXYPHXPTS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	229

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	first WW domain of human YAP	1
2	Smad1 doubly-phosphorilated derived peptide	2

Entities:

Entity 1, first WW domain of human YAP 40 residues - 4151.635 Da.

1	GLY	ALA	MET	GLU	ASP	VAL	PRO	LEU	PRO	ALA
2	GLY	TRP	GLU	MET	ALA	LYS	THR	SER	SER	GLY
3	GLN	ARG	TYR	PHE	LEU	ASN	HIS	ILE	ASP	GLN
4	THR	THR	THR	TRP	GLN	ASP	PRO	ARG	LYS	ALA

Entity 2, Smad1 doubly-phosphorilated derived peptide 9 residues - 1136.976 Da.

1

SER

TPO

TYR

PRO

HIS

SEP

PRO

THR

SER

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	H	isotropic	sample_conditions_1
2D 1H-1H TOCSY	H	isotropic	sample_conditions_1
3D CBCA(CO)NH	15N13C	isotropic	sample_conditions_1
3D HNCACB	15N13C	isotropic	sample_conditions_1
2D 1H-15N HSQC	15N	isotropic	sample_conditions_1

BMRB	17540 18499
PDB	1K9Q 1K9R 2LAX 2LAY 2LTW 4REX
DBJ	BAJ41471
EMBL	CAA56672
GB	EAW67012 EAW67014
REF	NP_001269026 NP_001269027 NP_001269028 NP_006097 XP_002799821
AlphaFold	Q9UFT8

BMRB Entry 17539

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	GLY	ALA	MET	GLU	ASP	VAL	PRO	LEU	PRO	ALA
2	GLY	TRP	GLU	MET	ALA	LYS	THR	SER	SER	GLY
3	GLN	ARG	TYR	PHE	LEU	ASN	HIS	ILE	ASP	GLN
4	THR	THR	THR	TRP	GLN	ASP	PRO	ARG	LYS	ALA

1	GLY	ALA	MET	GLU	ASP	VAL	PRO	LEU	PRO	ALA
2	GLY	TRP	GLU	MET	ALA	LYS	THR	SER	SER	GLY
3	GLN	ARG	TYR	PHE	LEU	ASN	HIS	ILE	ASP	GLN
4	THR	THR	THR	TRP	GLN	ASP	PRO	ARG	LYS	ALA

1	GLY	ALA	MET	GLU	ASP	VAL	PRO	LEU	PRO	ALA
2	GLY	TRP	GLU	MET	ALA	LYS	THR	SER	SER	GLY
3	GLN	ARG	TYR	PHE	LEU	ASN	HIS	ILE	ASP	GLN
4	THR	THR	THR	TRP	GLN	ASP	PRO	ARG	LYS	ALA