BMRB Entry 17537

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17537
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Title:
Solution structure of a Human minimembrane protein OST4
Deposition date:
2011-03-21
Original release date:
2011-06-03
Authors:
Gayen, Shovanlal; Kang, Congbao
Citation:

Citation: Gayen, Shovanlal; Kang, Congbao. "Solution structure of a human minimembrane protein Ost4, a subunit of the oligosaccharyltransferase complex."  Biochem. Biophys. Res. Commun. 409, 572-576 (2011).
PubMed: 21609714

Assembly members:

Assembly members:
Human minimembrane protein OST4, polymer, 37 residues, 4196.043 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Human minimembrane protein OST4: MITDVQLAIFANMLGVSLFL LVVLYHYVAVNNPKKQE

Data sets:
Data typeCount
1H chemical shifts202

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human minimembrane protein OST41

Entities:

Entity 1, Human minimembrane protein OST4 37 residues - 4196.043 Da.

1   METILETHRASPVALGLNLEUALAILEPHE
2   ALAASNMETLEUGLYVALSERLEUPHELEU
3   LEUVALVALLEUTYRHISTYRVALALAVAL
4   ASNASNPROLYSLYSGLNGLU

Samples:

sample_1: OST42 – 3 mM; CDC13 44%; Cd3OH 44%; H2O 12%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAC24992 BAC25041 BAC25115 BAC25288 BAC25588
GB AAH02177 AAH82316 AAI57815 ACH44975 ACH44976
REF NP_001128162 NP_001128163 NP_001128164 NP_001128165 NP_001185794
SP B0BLS0 P0C6T2 Q99LX8
TPG DAA24455
AlphaFold B0BLS0 P0C6T2 Q99LX8