BMRB Entry 17531

Name: Three-dimensional structure of the cyclotide Cter M
Published: 2011-05-27

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PDB ID: 2lam
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17531
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Three-dimensional structure of the cyclotide Cter M

Deposition date:

2011-03-16

Original release date:

2011-05-27

Authors:

Poth, Aaron; Colgrave, Michelle; Lyons, Russell; Daly, Norelle; Craik, David

Citation:

Citation: Poth, Aaron; Colgrave, Michelle; Lyons, Russell; Daly, Norelle; Craik, David. "From the Cover: Discovery of an unusual biosynthetic origin for circular proteins in legumes." Proc. Natl. Acad. Sci. U.S.A. 108, 10127-10132 (2011).
PubMed: 21593408

Assembly members:

Assembly members:
entity, polymer, 29 residues, 3084.605 Da.

Natural source:

Natural source: Common Name: Clitoria ternatea Taxonomy ID: 43366 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Clitoria ternatea

Experimental source:

Experimental source: Production method: chemical synthesis Vector: N/A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GLPTCGETCTLGTCYVPDCS CSWPICMKN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	174

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	circular proteins in legumes	1

Entities:

Entity 1, circular proteins in legumes 29 residues - 3084.605 Da.

1

GLY

LEU

PRO

THR

CYS

GLY

GLU

THR

CYS

THR

2

LEU

GLY

THR

CYS

TYR

VAL

PRO

ASP

CYS

SER

3

CYS

SER

TRP

PRO

ILE

CYS

MET

LYS

ASN

Samples:

sample_1: Cter M 1 mM

sample_conditions_1: ionic strength: . M; pH: 4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

XEASY, Bartels et al. - chemical shift assignment

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

Bruker Avance 900 MHz
Bruker Avance 600 MHz

PDB	2LAM
GB	AEB92229 AEK26404 ALL96779
SP	P86899
AlphaFold	P86899