BMRB Entry 17527
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17527
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Citation: Hiruma-Shimizu, Kazumi; Hosoguchi, Kensaku; Liu, Yan; Fujitani, Naoki; Ohta, Takashi; Hinou, Hiroshi; Matsushita, Takahiko; Shimizu, Hiroki; Feizi, Ten; Nishimura, Shin-ichiro. "Chemical synthesis, folding, and structural insights into O-fucosylated epidermal growth factor-like repeat 12 of mouse Notch-1 receptor." J. Am. Chem. Soc. 132, 14857-14865 (2010).
PubMed: 20883017
Assembly members:
NEUROGENIC_LOCUS_NOTCH_HOMOLOG_PROTEIN_1_EGF12_domain, polymer, 38 residues, Formula weight is not available
FUC, non-polymer, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: chemical synthesis Host organism: chemical snthesis Vector: chemical synthesis
Entity Sequences (FASTA):
NEUROGENIC_LOCUS_NOTCH_HOMOLOG_PROTEIN_1_EGF12_domain: DVNECISNPCQNDATCLDQI
GEFQCICMPGYEGVYCEI
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 253 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | EGF12 peptide | 1 |
| 2 | FUC | 2 |
Entities:
Entity 1, EGF12 peptide 38 residues - Formula weight is not available
The C terminus Isoleucine 38 id not free form from COOH but CONH2
| 1 | ASP | VAL | ASN | GLU | CYS | ILE | SER | ASN | PRO | CYS | ||||
| 2 | GLN | ASN | ASP | ALA | THR | CYS | LEU | ASP | GLN | ILE | ||||
| 3 | GLY | GLU | PHE | GLN | CYS | ILE | CYS | MET | PRO | GLY | ||||
| 4 | TYR | GLU | GLY | VAL | TYR | CYS | GLU | ILE |
Entity 2, FUC - Formula weight is not available
| 1 | FUC |
Samples:
sample_1: NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 1 0.67 mM; FUC 0.67 mM
sample2: NEUROGENIC LOCUS NOTCH HOMOLOG PROTEIN 1 0.67 mM; FUC 0.67 mM
sample_conditions_1: pH: 5.3; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample2 | isotropic | sample_conditions_1 |
Software:
NMRPipe v2.4, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, processing
NMR spectrometers:
- Bruker Avance 600 MHz