BMRB Entry 17524

Name: Solution NMR Structure of Mitotic checkpoint serine/threonine-protein kinase BUB1 N-terminal domain from Homo sapiens, Northeast Structural Genomics Consortium Target HR5460A
Published: 2011-05-09

Click here to enlarge.

PDB ID: 2lah
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17524
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution NMR Structure of Mitotic checkpoint serine/threonine-protein kinase BUB1 N-terminal domain from Homo sapiens, Northeast Structural Genomics Consortium Target HR5460A

Deposition date:

2011-03-14

Original release date:

2011-05-09

Authors:

Liu, G.; Xiao, R.; Lee, H.; Hamilton, K.; Acton, T.; Ciccosanti, C.; Everett, J.; Shastry, R.; Huang, Y.; Montelione, G.

Citation:

Citation: Liu, G.; Shastry, R.; Ciccosanti, C.; Hamilton, K.; Acton, T.; Xiao, R.; Everett, J.; Montelione, G.. "Northeast Structural Genomics Consortium Target HR5460A" To be published ., .-..

Assembly members:

Assembly members:
HR5460A, polymer, 160 residues, 19209.656 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 14-15C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HR5460A: MGHHHHHHSHMDTPENVLQM LEAHMQSYKGNDPLGEWERY IQWVEENFPENKEYLITLLE HLMKEFLDKKKYHNDPRFIS YCLKFAEYNSDLHQFFEFLY NHGIGTLSSPLYIAWAGHLE AQGELQHASAVLQRGIQNQA EPREFLQQQYRLFQTRLTET

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
RDCs
- RDC_list_1

Data type	Count
13C chemical shifts	695
15N chemical shifts	156
1H chemical shifts	1101
residual dipolar couplings	84

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HR5460A	1

Entities:

Entity 1, HR5460A 160 residues - 19209.656 Da.

1	MET	GLY	HIS	HIS	HIS	HIS	HIS	HIS	SER	HIS
2	MET	ASP	THR	PRO	GLU	ASN	VAL	LEU	GLN	MET
3	LEU	GLU	ALA	HIS	MET	GLN	SER	TYR	LYS	GLY
4	ASN	ASP	PRO	LEU	GLY	GLU	TRP	GLU	ARG	TYR
5	ILE	GLN	TRP	VAL	GLU	GLU	ASN	PHE	PRO	GLU
6	ASN	LYS	GLU	TYR	LEU	ILE	THR	LEU	LEU	GLU
7	HIS	LEU	MET	LYS	GLU	PHE	LEU	ASP	LYS	LYS
8	LYS	TYR	HIS	ASN	ASP	PRO	ARG	PHE	ILE	SER
9	TYR	CYS	LEU	LYS	PHE	ALA	GLU	TYR	ASN	SER
10	ASP	LEU	HIS	GLN	PHE	PHE	GLU	PHE	LEU	TYR
11	ASN	HIS	GLY	ILE	GLY	THR	LEU	SER	SER	PRO
12	LEU	TYR	ILE	ALA	TRP	ALA	GLY	HIS	LEU	GLU
13	ALA	GLN	GLY	GLU	LEU	GLN	HIS	ALA	SER	ALA
14	VAL	LEU	GLN	ARG	GLY	ILE	GLN	ASN	GLN	ALA
15	GLU	PRO	ARG	GLU	PHE	LEU	GLN	GLN	GLN	TYR
16	ARG	LEU	PHE	GLN	THR	ARG	LEU	THR	GLU	THR

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_NC	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_NC5	isotropic	sample_conditions_1
3D HNCO	sample_NC	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_NC	isotropic	sample_conditions_1
3D HNCACB	sample_NC	isotropic	sample_conditions_1
3D 1H-13C arom NOESY	sample_NC	isotropic	sample_conditions_1
3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY	sample_NC	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_NC5	isotropic	sample_conditions_1

PDB	2LAH 4A1G
DBJ	BAF83910
GB	AAB97855 AAC03122 AAC06259 AAC12729 AAD43675
REF	NP_001265546 NP_004327 XP_001142040 XP_002811811 XP_003804751
SP	O43683
AlphaFold	O43683

BMRB Entry 17524

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	GLY	HIS	HIS	HIS	HIS	HIS	HIS	SER	HIS
2	MET	ASP	THR	PRO	GLU	ASN	VAL	LEU	GLN	MET
3	LEU	GLU	ALA	HIS	MET	GLN	SER	TYR	LYS	GLY
4	ASN	ASP	PRO	LEU	GLY	GLU	TRP	GLU	ARG	TYR
5	ILE	GLN	TRP	VAL	GLU	GLU	ASN	PHE	PRO	GLU
6	ASN	LYS	GLU	TYR	LEU	ILE	THR	LEU	LEU	GLU
7	HIS	LEU	MET	LYS	GLU	PHE	LEU	ASP	LYS	LYS
8	LYS	TYR	HIS	ASN	ASP	PRO	ARG	PHE	ILE	SER
9	TYR	CYS	LEU	LYS	PHE	ALA	GLU	TYR	ASN	SER
10	ASP	LEU	HIS	GLN	PHE	PHE	GLU	PHE	LEU	TYR
11	ASN	HIS	GLY	ILE	GLY	THR	LEU	SER	SER	PRO
12	LEU	TYR	ILE	ALA	TRP	ALA	GLY	HIS	LEU	GLU
13	ALA	GLN	GLY	GLU	LEU	GLN	HIS	ALA	SER	ALA
14	VAL	LEU	GLN	ARG	GLY	ILE	GLN	ASN	GLN	ALA
15	GLU	PRO	ARG	GLU	PHE	LEU	GLN	GLN	GLN	TYR
16	ARG	LEU	PHE	GLN	THR	ARG	LEU	THR	GLU	THR

1	MET	GLY	HIS	HIS	HIS	HIS	HIS	HIS	SER	HIS
2	MET	ASP	THR	PRO	GLU	ASN	VAL	LEU	GLN	MET
3	LEU	GLU	ALA	HIS	MET	GLN	SER	TYR	LYS	GLY
4	ASN	ASP	PRO	LEU	GLY	GLU	TRP	GLU	ARG	TYR
5	ILE	GLN	TRP	VAL	GLU	GLU	ASN	PHE	PRO	GLU
6	ASN	LYS	GLU	TYR	LEU	ILE	THR	LEU	LEU	GLU
7	HIS	LEU	MET	LYS	GLU	PHE	LEU	ASP	LYS	LYS
8	LYS	TYR	HIS	ASN	ASP	PRO	ARG	PHE	ILE	SER
9	TYR	CYS	LEU	LYS	PHE	ALA	GLU	TYR	ASN	SER
10	ASP	LEU	HIS	GLN	PHE	PHE	GLU	PHE	LEU	TYR
11	ASN	HIS	GLY	ILE	GLY	THR	LEU	SER	SER	PRO
12	LEU	TYR	ILE	ALA	TRP	ALA	GLY	HIS	LEU	GLU
13	ALA	GLN	GLY	GLU	LEU	GLN	HIS	ALA	SER	ALA
14	VAL	LEU	GLN	ARG	GLY	ILE	GLN	ASN	GLN	ALA
15	GLU	PRO	ARG	GLU	PHE	LEU	GLN	GLN	GLN	TYR
16	ARG	LEU	PHE	GLN	THR	ARG	LEU	THR	GLU	THR

1	MET	GLY	HIS	HIS	HIS	HIS	HIS	HIS	SER	HIS
2	MET	ASP	THR	PRO	GLU	ASN	VAL	LEU	GLN	MET
3	LEU	GLU	ALA	HIS	MET	GLN	SER	TYR	LYS	GLY
4	ASN	ASP	PRO	LEU	GLY	GLU	TRP	GLU	ARG	TYR
5	ILE	GLN	TRP	VAL	GLU	GLU	ASN	PHE	PRO	GLU
6	ASN	LYS	GLU	TYR	LEU	ILE	THR	LEU	LEU	GLU
7	HIS	LEU	MET	LYS	GLU	PHE	LEU	ASP	LYS	LYS
8	LYS	TYR	HIS	ASN	ASP	PRO	ARG	PHE	ILE	SER
9	TYR	CYS	LEU	LYS	PHE	ALA	GLU	TYR	ASN	SER
10	ASP	LEU	HIS	GLN	PHE	PHE	GLU	PHE	LEU	TYR
11	ASN	HIS	GLY	ILE	GLY	THR	LEU	SER	SER	PRO
12	LEU	TYR	ILE	ALA	TRP	ALA	GLY	HIS	LEU	GLU
13	ALA	GLN	GLY	GLU	LEU	GLN	HIS	ALA	SER	ALA
14	VAL	LEU	GLN	ARG	GLY	ILE	GLN	ASN	GLN	ALA
15	GLU	PRO	ARG	GLU	PHE	LEU	GLN	GLN	GLN	TYR
16	ARG	LEU	PHE	GLN	THR	ARG	LEU	THR	GLU	THR