BMRB Entry 17520

Title:
Unmodified_ASL_Tyr   PubMed: 21782828
Deposition date:
2011-03-10
Original release date:
2011-08-02
Authors:
Denmon, Andria; Wang, Jiachen; Nikonowicz, Edward
Citation:

Citation: Denmon, Andria; Wang, Jiachen; Nikonowicz, Edward. "Conformation effects of base modification on the anticodon stem-loop of Bacillus subtilis tRNA(Tyr)."  J. Mol. Biol. 412, 285-303 (2011).

Assembly members:

Assembly members:
Unmod_tyrASL, polymer, 17 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: B. subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: cell free synthesis

Experimental source:

Natural source:   Common Name: B. subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Unmod_tyrASL: GGGGACUGUAAAUCCCC

Data sets:
Data typeCount
13C chemical shifts116
15N chemical shifts41
1H chemical shifts136
31P chemical shifts16

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Unmodified ASL_Tyr1

Entities:

Entity 1, Unmodified ASL_Tyr 17 residues - Formula weight is not available

1   GGGGACUGUA
2   AAUCCCC

Samples:

sample_1: Unmod_tyrASL, [U-100% 13C; U-100% 15N], 2.0 mM; potassium chloride 10 mM; potassium phosphate 10 mM; EDTA 0.02 mM; D2O 100%

sample_conditions_1: pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D HCNsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 31P-1Hsample_1isotropicsample_conditions_1
2D HCCH-RELAYsample_1isotropicsample_conditions_1
2D HCCH-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

VNMRJ, Varian - collection, processing

InsightII, Accelrys Software Inc. - data analysis

FELIX, Accelrys Software Inc. - chemical shift assignment, peak picking, processing

NMR spectrometers:

  • Varian INOVA 500 MHz