BMRB Entry 17514

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Leucine Zipper c-Jun.
Published: 2012-08-07

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17514

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Leucine Zipper c-Jun.

Deposition date:

2011-03-07

Original release date:

2012-08-07

Authors:

Nikolaev, Yaroslav; Pervushin, Konstantin

Citation:

Citation: Nikolaev, Yaroslav; Pervushin, Konstantin. "Structural basis of RNA binding by leucine zipper GCN4" Protein Sci. 21, 667-676 (2012).
PubMed: 22374868

Assembly members:

Assembly members:
LZ-cJun, polymer, 42 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LZ-cJun: GLERIARLEEKVKTLKAQNS ELASTANMLREQVAQLKQKV MN

Data sets:

assigned_chemical_shifts
- lzjun_coiled_coil_pH_7.1

Data type	Count
13C chemical shifts	78
15N chemical shifts	40
1H chemical shifts	40

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	LZ-cJun, 1	1
2	LZ-cJun, 2	1

Entities:

Entity 1, LZ-cJun, 1 42 residues - Formula weight is not available

1

GLY

LEU

GLU

ARG

ILE

ALA

ARG

LEU

GLU

2

LYS

VAL

LYS

THR

LEU

LYS

ALA

GLN

ASN

SER

3

GLU

LEU

ALA

SER

THR

ALA

ASN

MET

LEU

ARG

4

GLU

GLN

VAL

ALA

GLN

LEU

LYS

GLN

LYS

VAL

5

MET

ASN

Samples:

lzjun_NC: LZ-cJun, [U-13C; U-15N], 0.5 mM; KCl 85 mM

pH_7.1_295K: pH: 7.1; pressure: 1 atm; temperature: 295 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	lzjun_NC	isotropic	pH_7.1_295K
3D 1H-15N NOESY	lzjun_NC	isotropic	pH_7.1_295K

Software:

CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker AMX 800 MHz

BMRB	3394
PDB	1A02 1FOS 1JNM 1JUN 1S9K 1T2K 2H7H
DBJ	BAD98539 BAD98540 BAD98541 BAE20944 BAE22389
EMBL	CAA31236 CAA31252 CAA33553 CAA35041 CAA35084
GB	AAA37419 AAA42416 AAA48927 AAA49537 AAA59197
PRF	1404381A 1411298A 1411300A 2107314A
REF	NP_001026460 NP_001071295 NP_001185876 NP_001252779 NP_001274537
SP	O77627 P05411 P05412 P05627 P12981
TPG	DAA31168
AlphaFold	O77627 P05411 P05412 P05627 P12981